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Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation

Thermal adaptation is an extensively used intervention for enhancing or suppressing thermogenic and mitochondrial activity in adipose tissues. As such, it has been suggested as a potential lifestyle intervention for body weight maintenance. While the metabolic consequences of thermal acclimation are...

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Autores principales: Hadadi, Noushin, Spiljar, Martina, Steinbach, Karin, Çolakoğlu, Melis, Chevalier, Claire, Salinas, Gabriela, Merkler, Doron, Trajkovski, Mirko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113744/
https://www.ncbi.nlm.nih.gov/pubmed/35578890
http://dx.doi.org/10.7554/eLife.78556
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author Hadadi, Noushin
Spiljar, Martina
Steinbach, Karin
Çolakoğlu, Melis
Chevalier, Claire
Salinas, Gabriela
Merkler, Doron
Trajkovski, Mirko
author_facet Hadadi, Noushin
Spiljar, Martina
Steinbach, Karin
Çolakoğlu, Melis
Chevalier, Claire
Salinas, Gabriela
Merkler, Doron
Trajkovski, Mirko
author_sort Hadadi, Noushin
collection PubMed
description Thermal adaptation is an extensively used intervention for enhancing or suppressing thermogenic and mitochondrial activity in adipose tissues. As such, it has been suggested as a potential lifestyle intervention for body weight maintenance. While the metabolic consequences of thermal acclimation are not limited to the adipose tissues, the impact on the rest of the tissues in context of their gene expression profile remains unclear. Here, we provide a systematic characterization of the effects in a comparative multi-tissue RNA sequencing approach following exposure of mice to 10 °C, 22 °C, or 34 °C in a panel of organs consisting of spleen, bone marrow, spinal cord, brain, hypothalamus, ileum, liver, quadriceps, subcutaneous-, visceral- and brown adipose tissues. We highlight that transcriptional responses to temperature alterations exhibit a high degree of tissue-specificity both at the gene level and at GO enrichment gene sets, and show that the tissue-specificity is not directed by the distinct basic gene expression pattern exhibited by the various organs. Our study places the adaptation of individual tissues to different temperatures in a whole-organism framework and provides integrative transcriptional analysis necessary for understanding the temperature-mediated biological programming.
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spelling pubmed-91137442022-05-18 Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation Hadadi, Noushin Spiljar, Martina Steinbach, Karin Çolakoğlu, Melis Chevalier, Claire Salinas, Gabriela Merkler, Doron Trajkovski, Mirko eLife Computational and Systems Biology Thermal adaptation is an extensively used intervention for enhancing or suppressing thermogenic and mitochondrial activity in adipose tissues. As such, it has been suggested as a potential lifestyle intervention for body weight maintenance. While the metabolic consequences of thermal acclimation are not limited to the adipose tissues, the impact on the rest of the tissues in context of their gene expression profile remains unclear. Here, we provide a systematic characterization of the effects in a comparative multi-tissue RNA sequencing approach following exposure of mice to 10 °C, 22 °C, or 34 °C in a panel of organs consisting of spleen, bone marrow, spinal cord, brain, hypothalamus, ileum, liver, quadriceps, subcutaneous-, visceral- and brown adipose tissues. We highlight that transcriptional responses to temperature alterations exhibit a high degree of tissue-specificity both at the gene level and at GO enrichment gene sets, and show that the tissue-specificity is not directed by the distinct basic gene expression pattern exhibited by the various organs. Our study places the adaptation of individual tissues to different temperatures in a whole-organism framework and provides integrative transcriptional analysis necessary for understanding the temperature-mediated biological programming. eLife Sciences Publications, Ltd 2022-05-17 /pmc/articles/PMC9113744/ /pubmed/35578890 http://dx.doi.org/10.7554/eLife.78556 Text en © 2022, Hadadi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Computational and Systems Biology
Hadadi, Noushin
Spiljar, Martina
Steinbach, Karin
Çolakoğlu, Melis
Chevalier, Claire
Salinas, Gabriela
Merkler, Doron
Trajkovski, Mirko
Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
title Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
title_full Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
title_fullStr Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
title_full_unstemmed Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
title_short Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
title_sort comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
topic Computational and Systems Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113744/
https://www.ncbi.nlm.nih.gov/pubmed/35578890
http://dx.doi.org/10.7554/eLife.78556
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