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The effect of melatonin on the mouse ameloblast-lineage cell line ALCs
Melatonin plays a critical role in promoting the proliferation of osteoblasts and the growth and development of dental papilla cells. However, the effect and mechanism of melatonin on the growth and development of ALCs still need to be explored. CCK8 assay was used for the evaluation of cell numbers...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114102/ https://www.ncbi.nlm.nih.gov/pubmed/35581244 http://dx.doi.org/10.1038/s41598-022-11912-3 |
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author | Pan, Jing Ren, Qianhui Yang, Zhao Guo, Ying Kwon, Kubin Shen, Checheng Wang, Yueying Ji, Fang |
author_facet | Pan, Jing Ren, Qianhui Yang, Zhao Guo, Ying Kwon, Kubin Shen, Checheng Wang, Yueying Ji, Fang |
author_sort | Pan, Jing |
collection | PubMed |
description | Melatonin plays a critical role in promoting the proliferation of osteoblasts and the growth and development of dental papilla cells. However, the effect and mechanism of melatonin on the growth and development of ALCs still need to be explored. CCK8 assay was used for the evaluation of cell numbers. qRT-PCR was used to identify the differentially expressed genes in ALCs after melatonin treatment. The number and morphology of ALCs were investigated by confocal microscopy. Alkaline phosphatase assay and Alizarin red S staining were used for measuring mineralization. Then, we focused on observing the crucial factors of the signaling pathway by RNA-seq and qRT-PCR. Melatonin limited the cell number of ALCs in a dose-dependent manner and promoted the production of actin fibers. A high concentration of melatonin significantly promoted the mRNA levels of enamel matrix proteins and the formation of mineralized nodules. RNA-seq data showed that Wnt signaling pathway may be involved in the differentiation of ALCs under the influence of melatonin. This study suggests that melatonin plays a regulatory role in the cell number, differentiation, and mineralization of the ALCs, and then shows the relationship between the Wnt signaling pathway with the ALCs under melatonin. |
format | Online Article Text |
id | pubmed-9114102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91141022022-05-19 The effect of melatonin on the mouse ameloblast-lineage cell line ALCs Pan, Jing Ren, Qianhui Yang, Zhao Guo, Ying Kwon, Kubin Shen, Checheng Wang, Yueying Ji, Fang Sci Rep Article Melatonin plays a critical role in promoting the proliferation of osteoblasts and the growth and development of dental papilla cells. However, the effect and mechanism of melatonin on the growth and development of ALCs still need to be explored. CCK8 assay was used for the evaluation of cell numbers. qRT-PCR was used to identify the differentially expressed genes in ALCs after melatonin treatment. The number and morphology of ALCs were investigated by confocal microscopy. Alkaline phosphatase assay and Alizarin red S staining were used for measuring mineralization. Then, we focused on observing the crucial factors of the signaling pathway by RNA-seq and qRT-PCR. Melatonin limited the cell number of ALCs in a dose-dependent manner and promoted the production of actin fibers. A high concentration of melatonin significantly promoted the mRNA levels of enamel matrix proteins and the formation of mineralized nodules. RNA-seq data showed that Wnt signaling pathway may be involved in the differentiation of ALCs under the influence of melatonin. This study suggests that melatonin plays a regulatory role in the cell number, differentiation, and mineralization of the ALCs, and then shows the relationship between the Wnt signaling pathway with the ALCs under melatonin. Nature Publishing Group UK 2022-05-17 /pmc/articles/PMC9114102/ /pubmed/35581244 http://dx.doi.org/10.1038/s41598-022-11912-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pan, Jing Ren, Qianhui Yang, Zhao Guo, Ying Kwon, Kubin Shen, Checheng Wang, Yueying Ji, Fang The effect of melatonin on the mouse ameloblast-lineage cell line ALCs |
title | The effect of melatonin on the mouse ameloblast-lineage cell line ALCs |
title_full | The effect of melatonin on the mouse ameloblast-lineage cell line ALCs |
title_fullStr | The effect of melatonin on the mouse ameloblast-lineage cell line ALCs |
title_full_unstemmed | The effect of melatonin on the mouse ameloblast-lineage cell line ALCs |
title_short | The effect of melatonin on the mouse ameloblast-lineage cell line ALCs |
title_sort | effect of melatonin on the mouse ameloblast-lineage cell line alcs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114102/ https://www.ncbi.nlm.nih.gov/pubmed/35581244 http://dx.doi.org/10.1038/s41598-022-11912-3 |
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