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MAIT cell counts are associated with the risk of hospitalization in COPD

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by persistent airflow limitation associated with chronic inflammation in the airways. Mucosal-associated invariant T (MAIT) cells are unconventional, innate-like T cells highly abundant in mucosal tissues including the lung. W...

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Autores principales: Pincikova, Terezia, Parrot, Tiphaine, Hjelte, Lena, Högman, Marieann, Lisspers, Karin, Ställberg, Björn, Janson, Christer, Malinovschi, Andrei, Sandberg, Johan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114286/
https://www.ncbi.nlm.nih.gov/pubmed/35585629
http://dx.doi.org/10.1186/s12931-022-02045-2
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author Pincikova, Terezia
Parrot, Tiphaine
Hjelte, Lena
Högman, Marieann
Lisspers, Karin
Ställberg, Björn
Janson, Christer
Malinovschi, Andrei
Sandberg, Johan K.
author_facet Pincikova, Terezia
Parrot, Tiphaine
Hjelte, Lena
Högman, Marieann
Lisspers, Karin
Ställberg, Björn
Janson, Christer
Malinovschi, Andrei
Sandberg, Johan K.
author_sort Pincikova, Terezia
collection PubMed
description BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by persistent airflow limitation associated with chronic inflammation in the airways. Mucosal-associated invariant T (MAIT) cells are unconventional, innate-like T cells highly abundant in mucosal tissues including the lung. We hypothesized that the characteristics of MAIT cells in circulation may be prospectively associated with COPD morbidity. METHODS: COPD subjects (n = 61) from the Tools for Identifying Exacerbations (TIE) study were recruited when in stable condition. At study entry, forced expiratory volume in 1 s (FEV(1)) was measured and peripheral blood mononuclear cells were cryopreserved for later analysis by flow cytometry. Patients were followed for 3 years to record clinically meaningful outcomes. RESULTS: Patients who required hospitalization at one or more occasions during the 3-year follow-up (n = 21) had lower MAIT cell counts in peripheral blood at study inclusion, compared with patients who did not get hospitalized (p = 0.036). In contrast, hospitalized and never hospitalized patients did not differ in CD8 or CD4 T cell counts (p = 0.482 and p = 0.221, respectively). Moreover, MAIT cells in hospitalized subjects showed a more activated phenotype with higher CD38 expression (p = 0.014), and there was a trend towards higher LAG-3 expression (p = 0.052). Conventional CD4 and CD8 T cells were similar between the groups. Next we performed multi-variable logistic regression analysis with hospitalizations as dependent variable, and FEV(1), GOLD 2017 group, and quantity or activation of MAIT and conventional T cells as independent variables. MAIT cell count, CD38 expression on MAIT cells, and LAG-3 expression on both MAIT and CD8 T cells were all independently associated with the risk of hospitalization. CONCLUSIONS: These findings suggest that MAIT cells might reflect a novel, FEV(1)-independent immunological dimension in the complexity of COPD. The potential implication of MAIT cells in COPD pathogenesis and MAIT cells’ prognostic potential deserve further investigation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-022-02045-2.
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spelling pubmed-91142862022-05-18 MAIT cell counts are associated with the risk of hospitalization in COPD Pincikova, Terezia Parrot, Tiphaine Hjelte, Lena Högman, Marieann Lisspers, Karin Ställberg, Björn Janson, Christer Malinovschi, Andrei Sandberg, Johan K. Respir Res Research BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by persistent airflow limitation associated with chronic inflammation in the airways. Mucosal-associated invariant T (MAIT) cells are unconventional, innate-like T cells highly abundant in mucosal tissues including the lung. We hypothesized that the characteristics of MAIT cells in circulation may be prospectively associated with COPD morbidity. METHODS: COPD subjects (n = 61) from the Tools for Identifying Exacerbations (TIE) study were recruited when in stable condition. At study entry, forced expiratory volume in 1 s (FEV(1)) was measured and peripheral blood mononuclear cells were cryopreserved for later analysis by flow cytometry. Patients were followed for 3 years to record clinically meaningful outcomes. RESULTS: Patients who required hospitalization at one or more occasions during the 3-year follow-up (n = 21) had lower MAIT cell counts in peripheral blood at study inclusion, compared with patients who did not get hospitalized (p = 0.036). In contrast, hospitalized and never hospitalized patients did not differ in CD8 or CD4 T cell counts (p = 0.482 and p = 0.221, respectively). Moreover, MAIT cells in hospitalized subjects showed a more activated phenotype with higher CD38 expression (p = 0.014), and there was a trend towards higher LAG-3 expression (p = 0.052). Conventional CD4 and CD8 T cells were similar between the groups. Next we performed multi-variable logistic regression analysis with hospitalizations as dependent variable, and FEV(1), GOLD 2017 group, and quantity or activation of MAIT and conventional T cells as independent variables. MAIT cell count, CD38 expression on MAIT cells, and LAG-3 expression on both MAIT and CD8 T cells were all independently associated with the risk of hospitalization. CONCLUSIONS: These findings suggest that MAIT cells might reflect a novel, FEV(1)-independent immunological dimension in the complexity of COPD. The potential implication of MAIT cells in COPD pathogenesis and MAIT cells’ prognostic potential deserve further investigation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-022-02045-2. BioMed Central 2022-05-18 2022 /pmc/articles/PMC9114286/ /pubmed/35585629 http://dx.doi.org/10.1186/s12931-022-02045-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pincikova, Terezia
Parrot, Tiphaine
Hjelte, Lena
Högman, Marieann
Lisspers, Karin
Ställberg, Björn
Janson, Christer
Malinovschi, Andrei
Sandberg, Johan K.
MAIT cell counts are associated with the risk of hospitalization in COPD
title MAIT cell counts are associated with the risk of hospitalization in COPD
title_full MAIT cell counts are associated with the risk of hospitalization in COPD
title_fullStr MAIT cell counts are associated with the risk of hospitalization in COPD
title_full_unstemmed MAIT cell counts are associated with the risk of hospitalization in COPD
title_short MAIT cell counts are associated with the risk of hospitalization in COPD
title_sort mait cell counts are associated with the risk of hospitalization in copd
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114286/
https://www.ncbi.nlm.nih.gov/pubmed/35585629
http://dx.doi.org/10.1186/s12931-022-02045-2
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