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DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis

DEAD-box helicase 27 (DDX27) was previously identified as an important mediator during carcinogenesis, while its role in gastric cancer (GC) is not yet fully elucidated. Here, we aimed to investigate the mechanism and clinical significance of DDX27 in GC. Public datasets were analyzed to determine D...

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Autores principales: Jin, Yirong, Yang, Suzhen, Gao, Xiaoliang, Chen, Di, Luo, Tingting, Su, Song, Shi, Yanting, Yang, Gang, Dong, Lei, Liang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114675/
https://www.ncbi.nlm.nih.gov/pubmed/35601484
http://dx.doi.org/10.3389/fgene.2022.836199
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author Jin, Yirong
Yang, Suzhen
Gao, Xiaoliang
Chen, Di
Luo, Tingting
Su, Song
Shi, Yanting
Yang, Gang
Dong, Lei
Liang, Jie
author_facet Jin, Yirong
Yang, Suzhen
Gao, Xiaoliang
Chen, Di
Luo, Tingting
Su, Song
Shi, Yanting
Yang, Gang
Dong, Lei
Liang, Jie
author_sort Jin, Yirong
collection PubMed
description DEAD-box helicase 27 (DDX27) was previously identified as an important mediator during carcinogenesis, while its role in gastric cancer (GC) is not yet fully elucidated. Here, we aimed to investigate the mechanism and clinical significance of DDX27 in GC. Public datasets were analyzed to determine DDX27 expression profiling. The qRT-PCR, Western blot, and immunohistochemistry analyses were employed to investigate the DDX27 expression in GC cell lines and clinical samples. The role of DDX27 in GC metastasis was explored in vitro and in vivo. Mass spectrometry, RNA-seq, and alternative splicing analysis were conducted to demonstrate the DDX27-mediated molecular mechanisms in GC. We discovered that DDX27 was highly expressed in GCs, and a high level of DDX27 indicated poor prognosis. An increased DDX27 expression could promote GC metastasis, while DDX27 knockdown impaired GC aggressiveness. Mechanically, the LLP expression was significantly altered after DDX27 downregulation, and further results indicated that LPP may be regulated by DDX27 via alternative splicing. In summary, our study indicated that DDX27 contributed to GC malignant progression via a prometastatic DDX27/LPP/EMT regulatory axis.
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spelling pubmed-91146752022-05-19 DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis Jin, Yirong Yang, Suzhen Gao, Xiaoliang Chen, Di Luo, Tingting Su, Song Shi, Yanting Yang, Gang Dong, Lei Liang, Jie Front Genet Genetics DEAD-box helicase 27 (DDX27) was previously identified as an important mediator during carcinogenesis, while its role in gastric cancer (GC) is not yet fully elucidated. Here, we aimed to investigate the mechanism and clinical significance of DDX27 in GC. Public datasets were analyzed to determine DDX27 expression profiling. The qRT-PCR, Western blot, and immunohistochemistry analyses were employed to investigate the DDX27 expression in GC cell lines and clinical samples. The role of DDX27 in GC metastasis was explored in vitro and in vivo. Mass spectrometry, RNA-seq, and alternative splicing analysis were conducted to demonstrate the DDX27-mediated molecular mechanisms in GC. We discovered that DDX27 was highly expressed in GCs, and a high level of DDX27 indicated poor prognosis. An increased DDX27 expression could promote GC metastasis, while DDX27 knockdown impaired GC aggressiveness. Mechanically, the LLP expression was significantly altered after DDX27 downregulation, and further results indicated that LPP may be regulated by DDX27 via alternative splicing. In summary, our study indicated that DDX27 contributed to GC malignant progression via a prometastatic DDX27/LPP/EMT regulatory axis. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114675/ /pubmed/35601484 http://dx.doi.org/10.3389/fgene.2022.836199 Text en Copyright © 2022 Jin, Yang, Gao, Chen, Luo, Su, Shi, Yang, Dong and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Jin, Yirong
Yang, Suzhen
Gao, Xiaoliang
Chen, Di
Luo, Tingting
Su, Song
Shi, Yanting
Yang, Gang
Dong, Lei
Liang, Jie
DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis
title DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis
title_full DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis
title_fullStr DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis
title_full_unstemmed DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis
title_short DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis
title_sort dead-box helicase 27 triggers epithelial to mesenchymal transition by regulating alternative splicing of lipoma-preferred partner in gastric cancer metastasis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114675/
https://www.ncbi.nlm.nih.gov/pubmed/35601484
http://dx.doi.org/10.3389/fgene.2022.836199
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