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High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China

Borrelia burgdorferi sensu lato (BBSL), the causative agent of Lyme disease, is commonly found in wild and domestic mammals and ticks worldwide. In China, human cases of Borrelia burgdorferi infections have been identified across a wide geographic range including Yunnan Province, but few studies hav...

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Autores principales: He, Zhihai, Jiang, Baogui, Huang, Lin, Shao, Zongti, Zhang, Yun, Li, Yuqiong, Pu, Ennian, Duan, Xingde, Jiang, Hang, Wang, Jian, Yao, Mingguo, Wang, Fan, Bie, Shuangshuang, von Fricken, Michael E., Sun, Yi, Dong, Yi, Jiang, Jiafu, Du, Chunhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114702/
https://www.ncbi.nlm.nih.gov/pubmed/35602075
http://dx.doi.org/10.3389/fmicb.2022.876079
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author He, Zhihai
Jiang, Baogui
Huang, Lin
Shao, Zongti
Zhang, Yun
Li, Yuqiong
Pu, Ennian
Duan, Xingde
Jiang, Hang
Wang, Jian
Yao, Mingguo
Wang, Fan
Bie, Shuangshuang
von Fricken, Michael E.
Sun, Yi
Dong, Yi
Jiang, Jiafu
Du, Chunhong
author_facet He, Zhihai
Jiang, Baogui
Huang, Lin
Shao, Zongti
Zhang, Yun
Li, Yuqiong
Pu, Ennian
Duan, Xingde
Jiang, Hang
Wang, Jian
Yao, Mingguo
Wang, Fan
Bie, Shuangshuang
von Fricken, Michael E.
Sun, Yi
Dong, Yi
Jiang, Jiafu
Du, Chunhong
author_sort He, Zhihai
collection PubMed
description Borrelia burgdorferi sensu lato (BBSL), the causative agent of Lyme disease, is commonly found in wild and domestic mammals and ticks worldwide. In China, human cases of Borrelia burgdorferi infections have been identified across a wide geographic range including Yunnan Province, but few studies have examined BBSL in reservoirs and vectors in southwestern China. Here we conducted a thorough and broad-range investigation of BBSL in small mammals, domestic mammals, and ticks collected from 159 sample sites across 42 counties in Yunnan Province. DNA was extracted from spleen tissue of small mammals, blood from domestic mammals, and homogenized ticks. Nested PCR targeting the 5S-23S rRNA intergenic spacer gene of BBSL was used for screening, with amplicons sequenced directly and analyzed using a BLAST algorithm. A total of 8,478 samples were collected, which were composed of 5,044 mammals belonging to 68 species, 1,927 livestock belonging to five species, and 1, 507 ticks belonging to 14 species. BBSL was detected in 147 mammals (2.9%) from 30 different species, 20 of which represent the first reported detection in that species. A total of 52 (2.7%) livestock samples were positive for BBSL, with dogs having the highest detection rate (6.3%, 43/687), and 103 ticks (6.8%) tested positive with high prevalence in Ixodes granulatus (44.2%, 23/52), Haemaphysalis nepalensi (33.3%, 3/9) and Haemaphysalis kolonini (19.0%, 31/163). Sequence analysis revealed six genospecies of BBSL including B. afzelii, B. burgdorferi sensu stricto, B. japonica, B. garinii, B. sinica, and B. valaisiana. Significant differences in prevalence rates of BBSL were observed by species, landscape types, altitude, and season. Our findings indicate a wide distribution of multiple endemic BBSL genospecies based on a large-scale survey within Yunnan, which underline the need to expand surveillance efforts for human in southwestern China.
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spelling pubmed-91147022022-05-19 High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China He, Zhihai Jiang, Baogui Huang, Lin Shao, Zongti Zhang, Yun Li, Yuqiong Pu, Ennian Duan, Xingde Jiang, Hang Wang, Jian Yao, Mingguo Wang, Fan Bie, Shuangshuang von Fricken, Michael E. Sun, Yi Dong, Yi Jiang, Jiafu Du, Chunhong Front Microbiol Microbiology Borrelia burgdorferi sensu lato (BBSL), the causative agent of Lyme disease, is commonly found in wild and domestic mammals and ticks worldwide. In China, human cases of Borrelia burgdorferi infections have been identified across a wide geographic range including Yunnan Province, but few studies have examined BBSL in reservoirs and vectors in southwestern China. Here we conducted a thorough and broad-range investigation of BBSL in small mammals, domestic mammals, and ticks collected from 159 sample sites across 42 counties in Yunnan Province. DNA was extracted from spleen tissue of small mammals, blood from domestic mammals, and homogenized ticks. Nested PCR targeting the 5S-23S rRNA intergenic spacer gene of BBSL was used for screening, with amplicons sequenced directly and analyzed using a BLAST algorithm. A total of 8,478 samples were collected, which were composed of 5,044 mammals belonging to 68 species, 1,927 livestock belonging to five species, and 1, 507 ticks belonging to 14 species. BBSL was detected in 147 mammals (2.9%) from 30 different species, 20 of which represent the first reported detection in that species. A total of 52 (2.7%) livestock samples were positive for BBSL, with dogs having the highest detection rate (6.3%, 43/687), and 103 ticks (6.8%) tested positive with high prevalence in Ixodes granulatus (44.2%, 23/52), Haemaphysalis nepalensi (33.3%, 3/9) and Haemaphysalis kolonini (19.0%, 31/163). Sequence analysis revealed six genospecies of BBSL including B. afzelii, B. burgdorferi sensu stricto, B. japonica, B. garinii, B. sinica, and B. valaisiana. Significant differences in prevalence rates of BBSL were observed by species, landscape types, altitude, and season. Our findings indicate a wide distribution of multiple endemic BBSL genospecies based on a large-scale survey within Yunnan, which underline the need to expand surveillance efforts for human in southwestern China. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114702/ /pubmed/35602075 http://dx.doi.org/10.3389/fmicb.2022.876079 Text en Copyright © 2022 He, Jiang, Huang, Shao, Zhang, Li, Pu, Duan, Jiang, Wang, Yao, Wang, Bie, von Fricken, Sun, Dong, Jiang and Du. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
He, Zhihai
Jiang, Baogui
Huang, Lin
Shao, Zongti
Zhang, Yun
Li, Yuqiong
Pu, Ennian
Duan, Xingde
Jiang, Hang
Wang, Jian
Yao, Mingguo
Wang, Fan
Bie, Shuangshuang
von Fricken, Michael E.
Sun, Yi
Dong, Yi
Jiang, Jiafu
Du, Chunhong
High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China
title High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China
title_full High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China
title_fullStr High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China
title_full_unstemmed High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China
title_short High Diversity and Prevalence of Borrelia burgdorferi sensu lato in Wildlife Hosts, Domestic Animals, and Ticks in Yunnan Province, Southwestern China
title_sort high diversity and prevalence of borrelia burgdorferi sensu lato in wildlife hosts, domestic animals, and ticks in yunnan province, southwestern china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114702/
https://www.ncbi.nlm.nih.gov/pubmed/35602075
http://dx.doi.org/10.3389/fmicb.2022.876079
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