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The Foundation for Engineering a Pancreatic Islet Niche

Progress in diabetes research is hindered, in part, by deficiencies in current experimental systems to accurately model human pathophysiology and/or predict clinical outcomes. Engineering human-centric platforms that more closely mimic in vivo physiology, however, requires thoughtful and informed de...

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Autores principales: Patel, Smit N., Mathews, Clayton E., Chandler, Rachel, Stabler, Cherie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114707/
https://www.ncbi.nlm.nih.gov/pubmed/35600597
http://dx.doi.org/10.3389/fendo.2022.881525
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author Patel, Smit N.
Mathews, Clayton E.
Chandler, Rachel
Stabler, Cherie L.
author_facet Patel, Smit N.
Mathews, Clayton E.
Chandler, Rachel
Stabler, Cherie L.
author_sort Patel, Smit N.
collection PubMed
description Progress in diabetes research is hindered, in part, by deficiencies in current experimental systems to accurately model human pathophysiology and/or predict clinical outcomes. Engineering human-centric platforms that more closely mimic in vivo physiology, however, requires thoughtful and informed design. Summarizing our contemporary understanding of the unique and critical features of the pancreatic islet can inform engineering design criteria. Furthermore, a broad understanding of conventional experimental practices and their current advantages and limitations ensures that new models address key gaps. Improving beyond traditional cell culture, emerging platforms are combining diabetes-relevant cells within three-dimensional niches containing dynamic matrices and controlled fluidic flow. While highly promising, islet-on-a-chip prototypes must evolve their utility, adaptability, and adoptability to ensure broad and reproducible use. Here we propose a roadmap for engineers to craft biorelevant and accessible diabetes models. Concurrently, we seek to inspire biologists to leverage such tools to ask complex and nuanced questions. The progenies of such diabetes models should ultimately enable investigators to translate ambitious research expeditions from benchtop to the clinic.
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spelling pubmed-91147072022-05-19 The Foundation for Engineering a Pancreatic Islet Niche Patel, Smit N. Mathews, Clayton E. Chandler, Rachel Stabler, Cherie L. Front Endocrinol (Lausanne) Endocrinology Progress in diabetes research is hindered, in part, by deficiencies in current experimental systems to accurately model human pathophysiology and/or predict clinical outcomes. Engineering human-centric platforms that more closely mimic in vivo physiology, however, requires thoughtful and informed design. Summarizing our contemporary understanding of the unique and critical features of the pancreatic islet can inform engineering design criteria. Furthermore, a broad understanding of conventional experimental practices and their current advantages and limitations ensures that new models address key gaps. Improving beyond traditional cell culture, emerging platforms are combining diabetes-relevant cells within three-dimensional niches containing dynamic matrices and controlled fluidic flow. While highly promising, islet-on-a-chip prototypes must evolve their utility, adaptability, and adoptability to ensure broad and reproducible use. Here we propose a roadmap for engineers to craft biorelevant and accessible diabetes models. Concurrently, we seek to inspire biologists to leverage such tools to ask complex and nuanced questions. The progenies of such diabetes models should ultimately enable investigators to translate ambitious research expeditions from benchtop to the clinic. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114707/ /pubmed/35600597 http://dx.doi.org/10.3389/fendo.2022.881525 Text en Copyright © 2022 Patel, Mathews, Chandler and Stabler https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Patel, Smit N.
Mathews, Clayton E.
Chandler, Rachel
Stabler, Cherie L.
The Foundation for Engineering a Pancreatic Islet Niche
title The Foundation for Engineering a Pancreatic Islet Niche
title_full The Foundation for Engineering a Pancreatic Islet Niche
title_fullStr The Foundation for Engineering a Pancreatic Islet Niche
title_full_unstemmed The Foundation for Engineering a Pancreatic Islet Niche
title_short The Foundation for Engineering a Pancreatic Islet Niche
title_sort foundation for engineering a pancreatic islet niche
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114707/
https://www.ncbi.nlm.nih.gov/pubmed/35600597
http://dx.doi.org/10.3389/fendo.2022.881525
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