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Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing

The Trachypithecus leucocephalus (white-headed langur) is a highly endangered, karst-endemic primate species, inhabiting the karst limestone forest in Guangxi, Southwest China. How white-headed langurs adapted to karst limestone and special dietary remains unclear. It is the first time to study the...

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Autores principales: Que, Tengcheng, Pang, Xianwu, Huang, Hongli, Chen, Panyu, Wei, Yinfeng, Hua, Yiming, Liao, Hongjun, Wu, Jianbao, Li, Shousheng, Wu, Aiqiong, He, Meihong, Ruan, Xiangdong, Hu, Yanling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114771/
https://www.ncbi.nlm.nih.gov/pubmed/35601103
http://dx.doi.org/10.3389/fcimb.2022.872841
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author Que, Tengcheng
Pang, Xianwu
Huang, Hongli
Chen, Panyu
Wei, Yinfeng
Hua, Yiming
Liao, Hongjun
Wu, Jianbao
Li, Shousheng
Wu, Aiqiong
He, Meihong
Ruan, Xiangdong
Hu, Yanling
author_facet Que, Tengcheng
Pang, Xianwu
Huang, Hongli
Chen, Panyu
Wei, Yinfeng
Hua, Yiming
Liao, Hongjun
Wu, Jianbao
Li, Shousheng
Wu, Aiqiong
He, Meihong
Ruan, Xiangdong
Hu, Yanling
author_sort Que, Tengcheng
collection PubMed
description The Trachypithecus leucocephalus (white-headed langur) is a highly endangered, karst-endemic primate species, inhabiting the karst limestone forest in Guangxi, Southwest China. How white-headed langurs adapted to karst limestone and special dietary remains unclear. It is the first time to study the correlation between the gut microbiome of primates and special dietary, and environment in Guangxi. In the study, 150 fecal samples are collected from nine primates in Guangxi, China. Metagenomic sequencing is used to analyze and compare the gut microbiome composition and diversity between white-headed langurs and other primates. Our results indicate that white-headed langurs has a higher diversity of microbiome than other primates, and the key microbiome are phylum Firmicutes, class Clostridia, family Lachnospiraceae, and genera Clostridiates and Ruminococcus, which are related to the digestion and degradation of cellulose. Ten genera are significantly more abundant in white-headed langurs and François’ langur than in other primates, most of which are high-temperature microbiome. Functional analysis reveals that energy synthesis-related pathways and sugar metabolism-related pathways are less abundant in white-headed langurs and François’ langur than in other primates. This phenomenon could be an adaptation mechanism of leaf-eating primates to low-energy diet. The gut microbiome of white-headed langurs is related to diet and karst limestone environment. This study could serve as a reference to design conservation breeding, manage conservation units, and determine conservation priorities.
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spelling pubmed-91147712022-05-19 Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing Que, Tengcheng Pang, Xianwu Huang, Hongli Chen, Panyu Wei, Yinfeng Hua, Yiming Liao, Hongjun Wu, Jianbao Li, Shousheng Wu, Aiqiong He, Meihong Ruan, Xiangdong Hu, Yanling Front Cell Infect Microbiol Cellular and Infection Microbiology The Trachypithecus leucocephalus (white-headed langur) is a highly endangered, karst-endemic primate species, inhabiting the karst limestone forest in Guangxi, Southwest China. How white-headed langurs adapted to karst limestone and special dietary remains unclear. It is the first time to study the correlation between the gut microbiome of primates and special dietary, and environment in Guangxi. In the study, 150 fecal samples are collected from nine primates in Guangxi, China. Metagenomic sequencing is used to analyze and compare the gut microbiome composition and diversity between white-headed langurs and other primates. Our results indicate that white-headed langurs has a higher diversity of microbiome than other primates, and the key microbiome are phylum Firmicutes, class Clostridia, family Lachnospiraceae, and genera Clostridiates and Ruminococcus, which are related to the digestion and degradation of cellulose. Ten genera are significantly more abundant in white-headed langurs and François’ langur than in other primates, most of which are high-temperature microbiome. Functional analysis reveals that energy synthesis-related pathways and sugar metabolism-related pathways are less abundant in white-headed langurs and François’ langur than in other primates. This phenomenon could be an adaptation mechanism of leaf-eating primates to low-energy diet. The gut microbiome of white-headed langurs is related to diet and karst limestone environment. This study could serve as a reference to design conservation breeding, manage conservation units, and determine conservation priorities. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114771/ /pubmed/35601103 http://dx.doi.org/10.3389/fcimb.2022.872841 Text en Copyright © 2022 Que, Pang, Huang, Chen, Wei, Hua, Liao, Wu, Li, Wu, He, Ruan and Hu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Que, Tengcheng
Pang, Xianwu
Huang, Hongli
Chen, Panyu
Wei, Yinfeng
Hua, Yiming
Liao, Hongjun
Wu, Jianbao
Li, Shousheng
Wu, Aiqiong
He, Meihong
Ruan, Xiangdong
Hu, Yanling
Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing
title Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing
title_full Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing
title_fullStr Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing
title_full_unstemmed Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing
title_short Comparative Gut Microbiome in Trachypithecus leucocephalus and Other Primates in Guangxi, China, Based on Metagenome Sequencing
title_sort comparative gut microbiome in trachypithecus leucocephalus and other primates in guangxi, china, based on metagenome sequencing
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114771/
https://www.ncbi.nlm.nih.gov/pubmed/35601103
http://dx.doi.org/10.3389/fcimb.2022.872841
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