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Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation

CD4(+)Foxp3(+) regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced in vitro culture (iTreg) with different characteristics of suppressiveness, st...

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Autores principales: Chen, Weiqian, Huang, Weishan, Xue, Youqiu, Chen, Ye, Qian, Wenbin, Ma, Jilin, August, Avery, Wang, Julie, Zheng, Song Guo, Lin, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114772/
https://www.ncbi.nlm.nih.gov/pubmed/35603221
http://dx.doi.org/10.3389/fimmu.2022.900139
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author Chen, Weiqian
Huang, Weishan
Xue, Youqiu
Chen, Ye
Qian, Wenbin
Ma, Jilin
August, Avery
Wang, Julie
Zheng, Song Guo
Lin, Jin
author_facet Chen, Weiqian
Huang, Weishan
Xue, Youqiu
Chen, Ye
Qian, Wenbin
Ma, Jilin
August, Avery
Wang, Julie
Zheng, Song Guo
Lin, Jin
author_sort Chen, Weiqian
collection PubMed
description CD4(+)Foxp3(+) regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced in vitro culture (iTreg) with different characteristics of suppressiveness, stability, and plasticity. There is an abundance of published data on neuropilin-1 (Nrp-1) as a tTreg marker, but little data exist on iTreg. The fidelity of Nrp-1 as a tTreg marker and its role in iTreg remains to be explored. This study found that Nrp-1 was expressed by a subset of Foxp3(+)CD4(+)T cells in the central and peripheral lymphoid organs in intact mice, as well as in iTreg. Nrp-1(+)iTreg and Nrp-1(-)iTreg were adoptively transferred into a T cell-mediated colitis model to determine their ability to suppress inflammation. Differences in gene expression between Nrp-1(+) and Nrp-1(-)iTreg were analyzed by RNA sequencing. We demonstrated that the Nrp-1(+) subset of the iTreg exhibited enhanced suppressive function and stability compared to the Nrp-1(-) counterpart both in vivo and in vitro, partly depending on IL-10. We found that Nrp-1 is not an exclusive marker of tTreg, however, it is a biomarker identifying a new subset of iTreg with enhanced suppressive function, implicating a potential for Nrp-1(+)iTreg cell therapy for autoimmune and inflammatory diseases.
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spelling pubmed-91147722022-05-19 Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation Chen, Weiqian Huang, Weishan Xue, Youqiu Chen, Ye Qian, Wenbin Ma, Jilin August, Avery Wang, Julie Zheng, Song Guo Lin, Jin Front Immunol Immunology CD4(+)Foxp3(+) regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced in vitro culture (iTreg) with different characteristics of suppressiveness, stability, and plasticity. There is an abundance of published data on neuropilin-1 (Nrp-1) as a tTreg marker, but little data exist on iTreg. The fidelity of Nrp-1 as a tTreg marker and its role in iTreg remains to be explored. This study found that Nrp-1 was expressed by a subset of Foxp3(+)CD4(+)T cells in the central and peripheral lymphoid organs in intact mice, as well as in iTreg. Nrp-1(+)iTreg and Nrp-1(-)iTreg were adoptively transferred into a T cell-mediated colitis model to determine their ability to suppress inflammation. Differences in gene expression between Nrp-1(+) and Nrp-1(-)iTreg were analyzed by RNA sequencing. We demonstrated that the Nrp-1(+) subset of the iTreg exhibited enhanced suppressive function and stability compared to the Nrp-1(-) counterpart both in vivo and in vitro, partly depending on IL-10. We found that Nrp-1 is not an exclusive marker of tTreg, however, it is a biomarker identifying a new subset of iTreg with enhanced suppressive function, implicating a potential for Nrp-1(+)iTreg cell therapy for autoimmune and inflammatory diseases. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114772/ /pubmed/35603221 http://dx.doi.org/10.3389/fimmu.2022.900139 Text en Copyright © 2022 Chen, Huang, Xue, Chen, Qian, Ma, August, Wang, Zheng and Lin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chen, Weiqian
Huang, Weishan
Xue, Youqiu
Chen, Ye
Qian, Wenbin
Ma, Jilin
August, Avery
Wang, Julie
Zheng, Song Guo
Lin, Jin
Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
title Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
title_full Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
title_fullStr Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
title_full_unstemmed Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
title_short Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
title_sort neuropilin-1 identifies a new subpopulation of tgf-β-induced foxp3(+) regulatory t cells with potent suppressive function and enhanced stability during inflammation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114772/
https://www.ncbi.nlm.nih.gov/pubmed/35603221
http://dx.doi.org/10.3389/fimmu.2022.900139
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