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Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation
CD4(+)Foxp3(+) regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced in vitro culture (iTreg) with different characteristics of suppressiveness, st...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114772/ https://www.ncbi.nlm.nih.gov/pubmed/35603221 http://dx.doi.org/10.3389/fimmu.2022.900139 |
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author | Chen, Weiqian Huang, Weishan Xue, Youqiu Chen, Ye Qian, Wenbin Ma, Jilin August, Avery Wang, Julie Zheng, Song Guo Lin, Jin |
author_facet | Chen, Weiqian Huang, Weishan Xue, Youqiu Chen, Ye Qian, Wenbin Ma, Jilin August, Avery Wang, Julie Zheng, Song Guo Lin, Jin |
author_sort | Chen, Weiqian |
collection | PubMed |
description | CD4(+)Foxp3(+) regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced in vitro culture (iTreg) with different characteristics of suppressiveness, stability, and plasticity. There is an abundance of published data on neuropilin-1 (Nrp-1) as a tTreg marker, but little data exist on iTreg. The fidelity of Nrp-1 as a tTreg marker and its role in iTreg remains to be explored. This study found that Nrp-1 was expressed by a subset of Foxp3(+)CD4(+)T cells in the central and peripheral lymphoid organs in intact mice, as well as in iTreg. Nrp-1(+)iTreg and Nrp-1(-)iTreg were adoptively transferred into a T cell-mediated colitis model to determine their ability to suppress inflammation. Differences in gene expression between Nrp-1(+) and Nrp-1(-)iTreg were analyzed by RNA sequencing. We demonstrated that the Nrp-1(+) subset of the iTreg exhibited enhanced suppressive function and stability compared to the Nrp-1(-) counterpart both in vivo and in vitro, partly depending on IL-10. We found that Nrp-1 is not an exclusive marker of tTreg, however, it is a biomarker identifying a new subset of iTreg with enhanced suppressive function, implicating a potential for Nrp-1(+)iTreg cell therapy for autoimmune and inflammatory diseases. |
format | Online Article Text |
id | pubmed-9114772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91147722022-05-19 Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation Chen, Weiqian Huang, Weishan Xue, Youqiu Chen, Ye Qian, Wenbin Ma, Jilin August, Avery Wang, Julie Zheng, Song Guo Lin, Jin Front Immunol Immunology CD4(+)Foxp3(+) regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and inflammation. There are naturally-derived in the thymus (tTreg), generated extrathymically in the periphery (pTreg), and induced in vitro culture (iTreg) with different characteristics of suppressiveness, stability, and plasticity. There is an abundance of published data on neuropilin-1 (Nrp-1) as a tTreg marker, but little data exist on iTreg. The fidelity of Nrp-1 as a tTreg marker and its role in iTreg remains to be explored. This study found that Nrp-1 was expressed by a subset of Foxp3(+)CD4(+)T cells in the central and peripheral lymphoid organs in intact mice, as well as in iTreg. Nrp-1(+)iTreg and Nrp-1(-)iTreg were adoptively transferred into a T cell-mediated colitis model to determine their ability to suppress inflammation. Differences in gene expression between Nrp-1(+) and Nrp-1(-)iTreg were analyzed by RNA sequencing. We demonstrated that the Nrp-1(+) subset of the iTreg exhibited enhanced suppressive function and stability compared to the Nrp-1(-) counterpart both in vivo and in vitro, partly depending on IL-10. We found that Nrp-1 is not an exclusive marker of tTreg, however, it is a biomarker identifying a new subset of iTreg with enhanced suppressive function, implicating a potential for Nrp-1(+)iTreg cell therapy for autoimmune and inflammatory diseases. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114772/ /pubmed/35603221 http://dx.doi.org/10.3389/fimmu.2022.900139 Text en Copyright © 2022 Chen, Huang, Xue, Chen, Qian, Ma, August, Wang, Zheng and Lin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Chen, Weiqian Huang, Weishan Xue, Youqiu Chen, Ye Qian, Wenbin Ma, Jilin August, Avery Wang, Julie Zheng, Song Guo Lin, Jin Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation |
title | Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation |
title_full | Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation |
title_fullStr | Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation |
title_full_unstemmed | Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation |
title_short | Neuropilin-1 Identifies a New Subpopulation of TGF-β-Induced Foxp3(+) Regulatory T Cells With Potent Suppressive Function and Enhanced Stability During Inflammation |
title_sort | neuropilin-1 identifies a new subpopulation of tgf-β-induced foxp3(+) regulatory t cells with potent suppressive function and enhanced stability during inflammation |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114772/ https://www.ncbi.nlm.nih.gov/pubmed/35603221 http://dx.doi.org/10.3389/fimmu.2022.900139 |
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