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Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis

Pathological correlates of potential autoimmune gastritis (AIG), defined by anti-parietal cell antibody (PCA) positivity in the absence of gastric atrophy, have never been described. We herein aimed to assess intraepithelial lymphocyte (IEL) infiltration in gastric corpus of AIG patients. From 2000...

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Autores principales: Lenti, Marco Vincenzo, Vanoli, Alessandro, Miceli, Emanuela, Arpa, Giovanni, Di Stefano, Michele, Soriano, Simone, Capuano, Francesca, Gentile, Antonella, Aronico, Nicola, Coppola, Luigi, Pasini, Alessandra, Luinetti, Ombretta, Mauro, Aurelio, Paulli, Marco, Klersy, Catherine, Corazza, Gino Roberto, Di Sabatino, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114815/
https://www.ncbi.nlm.nih.gov/pubmed/35603187
http://dx.doi.org/10.3389/fimmu.2022.866167
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author Lenti, Marco Vincenzo
Vanoli, Alessandro
Miceli, Emanuela
Arpa, Giovanni
Di Stefano, Michele
Soriano, Simone
Capuano, Francesca
Gentile, Antonella
Aronico, Nicola
Coppola, Luigi
Pasini, Alessandra
Luinetti, Ombretta
Mauro, Aurelio
Paulli, Marco
Klersy, Catherine
Corazza, Gino Roberto
Di Sabatino, Antonio
author_facet Lenti, Marco Vincenzo
Vanoli, Alessandro
Miceli, Emanuela
Arpa, Giovanni
Di Stefano, Michele
Soriano, Simone
Capuano, Francesca
Gentile, Antonella
Aronico, Nicola
Coppola, Luigi
Pasini, Alessandra
Luinetti, Ombretta
Mauro, Aurelio
Paulli, Marco
Klersy, Catherine
Corazza, Gino Roberto
Di Sabatino, Antonio
author_sort Lenti, Marco Vincenzo
collection PubMed
description Pathological correlates of potential autoimmune gastritis (AIG), defined by anti-parietal cell antibody (PCA) positivity in the absence of gastric atrophy, have never been described. We herein aimed to assess intraepithelial lymphocyte (IEL) infiltration in gastric corpus of AIG patients. From 2000 to 2021, among 53 potential AIG patients, we focused on nine (median age 61 years, IQR 53-82; four females) who subsequently developed overt AIG. IEL infiltration of the oxyntic mucosa was assessed before and after developing overt AIG by measuring deep and superficial CD3+ IEL. AIG patients with different degrees of corpus atrophy, healthy controls (HC), active H. pylori gastritis, celiac disease (CD), and Hashimoto’s thyroiditis patients were included as controls. Of note, deep, but not superficial, CD3+ IEL count was higher (p<0.001) in potential AIG compared to HC and H. pylori gastritis. Deep CD3+ IEL infiltration did not change before or after the evolution into atrophy (median 9.6, IQR 8.8-12.4, vs 11.3, IQR 9.4-12.9). No difference was found in deep CD3+ IEL infiltration among potential, mild, and severe AIG, and compared to Hashimoto’s thyroiditis or CD. A deep CD3+ IEL cut-off of >7/100 epithelial cells allowed discrimination of any AIG stage and severity (AUC=0.842). We conclude that an increased deep CD3+ IEL infiltration of the oxyntic mucosa could represent a marker of potential AIG. Prospective studies including a larger number of potential AIG patients are needed.
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spelling pubmed-91148152022-05-19 Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis Lenti, Marco Vincenzo Vanoli, Alessandro Miceli, Emanuela Arpa, Giovanni Di Stefano, Michele Soriano, Simone Capuano, Francesca Gentile, Antonella Aronico, Nicola Coppola, Luigi Pasini, Alessandra Luinetti, Ombretta Mauro, Aurelio Paulli, Marco Klersy, Catherine Corazza, Gino Roberto Di Sabatino, Antonio Front Immunol Immunology Pathological correlates of potential autoimmune gastritis (AIG), defined by anti-parietal cell antibody (PCA) positivity in the absence of gastric atrophy, have never been described. We herein aimed to assess intraepithelial lymphocyte (IEL) infiltration in gastric corpus of AIG patients. From 2000 to 2021, among 53 potential AIG patients, we focused on nine (median age 61 years, IQR 53-82; four females) who subsequently developed overt AIG. IEL infiltration of the oxyntic mucosa was assessed before and after developing overt AIG by measuring deep and superficial CD3+ IEL. AIG patients with different degrees of corpus atrophy, healthy controls (HC), active H. pylori gastritis, celiac disease (CD), and Hashimoto’s thyroiditis patients were included as controls. Of note, deep, but not superficial, CD3+ IEL count was higher (p<0.001) in potential AIG compared to HC and H. pylori gastritis. Deep CD3+ IEL infiltration did not change before or after the evolution into atrophy (median 9.6, IQR 8.8-12.4, vs 11.3, IQR 9.4-12.9). No difference was found in deep CD3+ IEL infiltration among potential, mild, and severe AIG, and compared to Hashimoto’s thyroiditis or CD. A deep CD3+ IEL cut-off of >7/100 epithelial cells allowed discrimination of any AIG stage and severity (AUC=0.842). We conclude that an increased deep CD3+ IEL infiltration of the oxyntic mucosa could represent a marker of potential AIG. Prospective studies including a larger number of potential AIG patients are needed. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9114815/ /pubmed/35603187 http://dx.doi.org/10.3389/fimmu.2022.866167 Text en Copyright © 2022 Lenti, Vanoli, Miceli, Arpa, Di Stefano, Soriano, Capuano, Gentile, Aronico, Coppola, Pasini, Luinetti, Mauro, Paulli, Klersy, Corazza and Di Sabatino https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lenti, Marco Vincenzo
Vanoli, Alessandro
Miceli, Emanuela
Arpa, Giovanni
Di Stefano, Michele
Soriano, Simone
Capuano, Francesca
Gentile, Antonella
Aronico, Nicola
Coppola, Luigi
Pasini, Alessandra
Luinetti, Ombretta
Mauro, Aurelio
Paulli, Marco
Klersy, Catherine
Corazza, Gino Roberto
Di Sabatino, Antonio
Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis
title Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis
title_full Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis
title_fullStr Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis
title_full_unstemmed Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis
title_short Increase of Deep Intraepithelial Lymphocytes in the Oxyntic Mucosa of Patients With Potential and Overt Autoimmune Gastritis
title_sort increase of deep intraepithelial lymphocytes in the oxyntic mucosa of patients with potential and overt autoimmune gastritis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114815/
https://www.ncbi.nlm.nih.gov/pubmed/35603187
http://dx.doi.org/10.3389/fimmu.2022.866167
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