The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons

The Q system allows for conditional gene expression in several organisms, including C. elegans . We aimed to apply this system in C. elegans neurons to obtain temporally-resolved, tissue-specific expression of a fluorescent reporter. We report that, in our hands, there is undesired expression of the...

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Detalles Bibliográficos
Autores principales: Driesschaert, Brecht, Temmerman, Liesbet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Negative Result
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115586/
https://www.ncbi.nlm.nih.gov/pubmed/35601753
http://dx.doi.org/10.17912/micropub.biology.000573
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author Driesschaert, Brecht
Temmerman, Liesbet
author_facet Driesschaert, Brecht
Temmerman, Liesbet
author_sort Driesschaert, Brecht
collection PubMed
description The Q system allows for conditional gene expression in several organisms, including C. elegans . We aimed to apply this system in C. elegans neurons to obtain temporally-resolved, tissue-specific expression of a fluorescent reporter. We report that, in our hands, there is undesired expression of the reporter in conditions where expression is supposed to be repressed. In addition, in this setup, the signal‑to‑noise ratio of the Q system is unfavorable. We conclude that the Q system is far from optimal in the C. elegans nervous system, and advise cautious use.
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spelling pubmed-91155862022-05-19 The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons Driesschaert, Brecht Temmerman, Liesbet MicroPubl Biol Negative Result The Q system allows for conditional gene expression in several organisms, including C. elegans . We aimed to apply this system in C. elegans neurons to obtain temporally-resolved, tissue-specific expression of a fluorescent reporter. We report that, in our hands, there is undesired expression of the reporter in conditions where expression is supposed to be repressed. In addition, in this setup, the signal‑to‑noise ratio of the Q system is unfavorable. We conclude that the Q system is far from optimal in the C. elegans nervous system, and advise cautious use. Caltech Library 2022-05-17 /pmc/articles/PMC9115586/ /pubmed/35601753 http://dx.doi.org/10.17912/micropub.biology.000573 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Negative Result
Driesschaert, Brecht
Temmerman, Liesbet
The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons
title The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons
title_full The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons
title_fullStr The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons
title_full_unstemmed The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons
title_short The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons
title_sort q system for conditional gene expression is leaky and lacks dynamic range in c. elegans neurons
topic Negative Result
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115586/
https://www.ncbi.nlm.nih.gov/pubmed/35601753
http://dx.doi.org/10.17912/micropub.biology.000573
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