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TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis

Thyroid hormone receptor interactor 13 (TRIP13) plays a crucial role in poor prognosis and chemotherapy resistance of cancer patients. This present study is aimed at investigating the role of high expression of TRIP13 inducing nedaplatin (NDP) resistance in esophageal squamous cell carcinoma (ESCC)...

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Autores principales: Zhang, Lin-Ting, Ke, Li-Xin, Wu, Xin-Yi, Tian, Hui-Ting, Deng, Hua-Zhen, Xu, Li-Yan, Li, En-Min, Long, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115607/
https://www.ncbi.nlm.nih.gov/pubmed/35601150
http://dx.doi.org/10.1155/2022/7295458
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author Zhang, Lin-Ting
Ke, Li-Xin
Wu, Xin-Yi
Tian, Hui-Ting
Deng, Hua-Zhen
Xu, Li-Yan
Li, En-Min
Long, Lin
author_facet Zhang, Lin-Ting
Ke, Li-Xin
Wu, Xin-Yi
Tian, Hui-Ting
Deng, Hua-Zhen
Xu, Li-Yan
Li, En-Min
Long, Lin
author_sort Zhang, Lin-Ting
collection PubMed
description Thyroid hormone receptor interactor 13 (TRIP13) plays a crucial role in poor prognosis and chemotherapy resistance of cancer patients. This present study is aimed at investigating the role of high expression of TRIP13 inducing nedaplatin (NDP) resistance in esophageal squamous cell carcinoma (ESCC) cells. High expression of TRIP13 promoted the proliferation and migration of ESCC cells performed by MTS assay, colony formation assay, wound healing assay, and transwell assay. High TRIP13 expression induced NDP resistance to ESCC based on the cell proliferation promoting/inhibition rate and cell migration promoting/inhibition rate analysis, flow cytometry assay of apoptotic subpopulations with a combination of Annexin V-FITC and propidium iodide, and Western blot analysis downregulating cleaved PARP, γH2A.X, cleaved caspase-3, and Bax and upregulating Bcl-2 expression. This study indicated that high expression of TRIP13 promoted proliferation and migration of ESCC cells and induced NDP resistance via enhancing repair of DNA damage and inhibiting apoptosis. This will provide a preliminary reference for the clinical use of NDP in ESCC treatment.
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spelling pubmed-91156072022-05-19 TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis Zhang, Lin-Ting Ke, Li-Xin Wu, Xin-Yi Tian, Hui-Ting Deng, Hua-Zhen Xu, Li-Yan Li, En-Min Long, Lin Biomed Res Int Research Article Thyroid hormone receptor interactor 13 (TRIP13) plays a crucial role in poor prognosis and chemotherapy resistance of cancer patients. This present study is aimed at investigating the role of high expression of TRIP13 inducing nedaplatin (NDP) resistance in esophageal squamous cell carcinoma (ESCC) cells. High expression of TRIP13 promoted the proliferation and migration of ESCC cells performed by MTS assay, colony formation assay, wound healing assay, and transwell assay. High TRIP13 expression induced NDP resistance to ESCC based on the cell proliferation promoting/inhibition rate and cell migration promoting/inhibition rate analysis, flow cytometry assay of apoptotic subpopulations with a combination of Annexin V-FITC and propidium iodide, and Western blot analysis downregulating cleaved PARP, γH2A.X, cleaved caspase-3, and Bax and upregulating Bcl-2 expression. This study indicated that high expression of TRIP13 promoted proliferation and migration of ESCC cells and induced NDP resistance via enhancing repair of DNA damage and inhibiting apoptosis. This will provide a preliminary reference for the clinical use of NDP in ESCC treatment. Hindawi 2022-05-10 /pmc/articles/PMC9115607/ /pubmed/35601150 http://dx.doi.org/10.1155/2022/7295458 Text en Copyright © 2022 Lin-Ting Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Lin-Ting
Ke, Li-Xin
Wu, Xin-Yi
Tian, Hui-Ting
Deng, Hua-Zhen
Xu, Li-Yan
Li, En-Min
Long, Lin
TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis
title TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis
title_full TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis
title_fullStr TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis
title_full_unstemmed TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis
title_short TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis
title_sort trip13 induces nedaplatin resistance in esophageal squamous cell carcinoma by enhancing repair of dna damage and inhibiting apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115607/
https://www.ncbi.nlm.nih.gov/pubmed/35601150
http://dx.doi.org/10.1155/2022/7295458
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