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Development of a stem cell spheroid‐laden patch with high retention at skin wound site

Mesenchymal stem cells such as human adipose tissue‐derived stem cells (hADSCs) have been used as a representative therapeutic agent for tissue regeneration because of their high proliferation and paracrine factor‐secreting abilities. However, certain points regarding conventional ADSC delivery syst...

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Autores principales: Jeong, Gun‐Jae, Im, Gwang‐Bum, Lee, Tae‐Jin, Kim, Sung‐Won, Jeon, Hye Ran, Lee, Dong‐Hyun, Baik, Sangyul, Pang, Changhyun, Kim, Tae‐Hyung, Kim, Dong‐Ik, Jang, Young Charles, Bhang, Suk Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115685/
https://www.ncbi.nlm.nih.gov/pubmed/35600658
http://dx.doi.org/10.1002/btm2.10279
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author Jeong, Gun‐Jae
Im, Gwang‐Bum
Lee, Tae‐Jin
Kim, Sung‐Won
Jeon, Hye Ran
Lee, Dong‐Hyun
Baik, Sangyul
Pang, Changhyun
Kim, Tae‐Hyung
Kim, Dong‐Ik
Jang, Young Charles
Bhang, Suk Ho
author_facet Jeong, Gun‐Jae
Im, Gwang‐Bum
Lee, Tae‐Jin
Kim, Sung‐Won
Jeon, Hye Ran
Lee, Dong‐Hyun
Baik, Sangyul
Pang, Changhyun
Kim, Tae‐Hyung
Kim, Dong‐Ik
Jang, Young Charles
Bhang, Suk Ho
author_sort Jeong, Gun‐Jae
collection PubMed
description Mesenchymal stem cells such as human adipose tissue‐derived stem cells (hADSCs) have been used as a representative therapeutic agent for tissue regeneration because of their high proliferation and paracrine factor‐secreting abilities. However, certain points regarding conventional ADSC delivery systems, such as low cell density, secreted cytokine levels, and cell viability, still need to be addressed for treating severe wounds. In this study, we developed a three‐dimensional (3D) cavity‐structured stem cell‐laden system for overdense delivery of cells into severe wound sites. Our system includes a hydrophobic surface and cavities that can enhance the efficiency of cell delivery to the wound site. In particular, the cavities in the system facilitate hADSC spheroid formation, increasing therapeutic growth factor expression compared with 2D cultured cells. Our hADSC spheroid‐loaded patch exhibited remarkably improved cell localization at the wound site and dramatic therapeutic efficacy compared to the conventional cell injection method. Taken together, the hADSC spheroid delivery system focused on cell delivery, and stem cell homing effect at the wound site showed a significantly enhanced wound healing effect. By overcoming the limitations of conventional cell delivery methods, our overdense cell delivery system can contribute to biomedical and clinical applications.
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spelling pubmed-91156852022-05-20 Development of a stem cell spheroid‐laden patch with high retention at skin wound site Jeong, Gun‐Jae Im, Gwang‐Bum Lee, Tae‐Jin Kim, Sung‐Won Jeon, Hye Ran Lee, Dong‐Hyun Baik, Sangyul Pang, Changhyun Kim, Tae‐Hyung Kim, Dong‐Ik Jang, Young Charles Bhang, Suk Ho Bioeng Transl Med Research Articles Mesenchymal stem cells such as human adipose tissue‐derived stem cells (hADSCs) have been used as a representative therapeutic agent for tissue regeneration because of their high proliferation and paracrine factor‐secreting abilities. However, certain points regarding conventional ADSC delivery systems, such as low cell density, secreted cytokine levels, and cell viability, still need to be addressed for treating severe wounds. In this study, we developed a three‐dimensional (3D) cavity‐structured stem cell‐laden system for overdense delivery of cells into severe wound sites. Our system includes a hydrophobic surface and cavities that can enhance the efficiency of cell delivery to the wound site. In particular, the cavities in the system facilitate hADSC spheroid formation, increasing therapeutic growth factor expression compared with 2D cultured cells. Our hADSC spheroid‐loaded patch exhibited remarkably improved cell localization at the wound site and dramatic therapeutic efficacy compared to the conventional cell injection method. Taken together, the hADSC spheroid delivery system focused on cell delivery, and stem cell homing effect at the wound site showed a significantly enhanced wound healing effect. By overcoming the limitations of conventional cell delivery methods, our overdense cell delivery system can contribute to biomedical and clinical applications. John Wiley & Sons, Inc. 2021-12-28 /pmc/articles/PMC9115685/ /pubmed/35600658 http://dx.doi.org/10.1002/btm2.10279 Text en © 2021 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of The American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Jeong, Gun‐Jae
Im, Gwang‐Bum
Lee, Tae‐Jin
Kim, Sung‐Won
Jeon, Hye Ran
Lee, Dong‐Hyun
Baik, Sangyul
Pang, Changhyun
Kim, Tae‐Hyung
Kim, Dong‐Ik
Jang, Young Charles
Bhang, Suk Ho
Development of a stem cell spheroid‐laden patch with high retention at skin wound site
title Development of a stem cell spheroid‐laden patch with high retention at skin wound site
title_full Development of a stem cell spheroid‐laden patch with high retention at skin wound site
title_fullStr Development of a stem cell spheroid‐laden patch with high retention at skin wound site
title_full_unstemmed Development of a stem cell spheroid‐laden patch with high retention at skin wound site
title_short Development of a stem cell spheroid‐laden patch with high retention at skin wound site
title_sort development of a stem cell spheroid‐laden patch with high retention at skin wound site
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115685/
https://www.ncbi.nlm.nih.gov/pubmed/35600658
http://dx.doi.org/10.1002/btm2.10279
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