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Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis

The removal of particles using fluoropolymer-based membrane filters is usually done so to prolong the life span of an analytical column, prevent hardware damage, and reduce signal suppression. Ironically, these membrane filters tend to leach impurities into the samples as the samples are filtered th...

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Autores principales: Puah, Perng Yang, Herng Lee, Dexter Jiunn, Mak, Ken Hing, Ang, Hui Jun, Chen, Hsing-Chang, Moh, Pak Yan, Fong, Siat Yee, Ling, Yee Soon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116114/
https://www.ncbi.nlm.nih.gov/pubmed/35702663
http://dx.doi.org/10.1039/c9ra06198c
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author Puah, Perng Yang
Herng Lee, Dexter Jiunn
Mak, Ken Hing
Ang, Hui Jun
Chen, Hsing-Chang
Moh, Pak Yan
Fong, Siat Yee
Ling, Yee Soon
author_facet Puah, Perng Yang
Herng Lee, Dexter Jiunn
Mak, Ken Hing
Ang, Hui Jun
Chen, Hsing-Chang
Moh, Pak Yan
Fong, Siat Yee
Ling, Yee Soon
author_sort Puah, Perng Yang
collection PubMed
description The removal of particles using fluoropolymer-based membrane filters is usually done so to prolong the life span of an analytical column, prevent hardware damage, and reduce signal suppression. Ironically, these membrane filters tend to leach impurities into the samples as the samples are filtered through them. These impurities have the potential to affect the researcher's interpretation in high-throughput, non-targeted analysis. In this study, extractable impurities from different brands of fluoropolymer-based membrane filters present in the filtrate filtered using the said filters were investigated. The results demonstrated that different brand membrane filters and materials tend to elute vastly different numbers of impurities. There were instances whereby the extractable impurities persisted in both the membrane filter and the filtrate despite the filter being pre-conditioned (up to 3 times). Principle component analysis revealed that filtrates at different purge intervals are distant from the unfiltered samples. Pre-conditioning of the PTFE membrane filters could potentially reduce the number of extractable impurities across the tested brands. PVDF filtrates, however, tend to co-cluster with their respective brands, thus suggesting that dissimilarity persists in brands following conditioning. As such, pre-conditioning of the PTFE membrane filters should be encouraged so as to reduce false positive results, while the use of PVDF membrane filters for mass-spectrometry-based untargeted analysis is not advisable as extractable impurities would still persist after 3 rounds of conditioning. Neither the use of different filter brands, nor the use of different filter materials in a sample batch are encouraged as different membrane materials or brands could potentially elute varying impurities.
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spelling pubmed-91161142022-06-13 Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis Puah, Perng Yang Herng Lee, Dexter Jiunn Mak, Ken Hing Ang, Hui Jun Chen, Hsing-Chang Moh, Pak Yan Fong, Siat Yee Ling, Yee Soon RSC Adv Chemistry The removal of particles using fluoropolymer-based membrane filters is usually done so to prolong the life span of an analytical column, prevent hardware damage, and reduce signal suppression. Ironically, these membrane filters tend to leach impurities into the samples as the samples are filtered through them. These impurities have the potential to affect the researcher's interpretation in high-throughput, non-targeted analysis. In this study, extractable impurities from different brands of fluoropolymer-based membrane filters present in the filtrate filtered using the said filters were investigated. The results demonstrated that different brand membrane filters and materials tend to elute vastly different numbers of impurities. There were instances whereby the extractable impurities persisted in both the membrane filter and the filtrate despite the filter being pre-conditioned (up to 3 times). Principle component analysis revealed that filtrates at different purge intervals are distant from the unfiltered samples. Pre-conditioning of the PTFE membrane filters could potentially reduce the number of extractable impurities across the tested brands. PVDF filtrates, however, tend to co-cluster with their respective brands, thus suggesting that dissimilarity persists in brands following conditioning. As such, pre-conditioning of the PTFE membrane filters should be encouraged so as to reduce false positive results, while the use of PVDF membrane filters for mass-spectrometry-based untargeted analysis is not advisable as extractable impurities would still persist after 3 rounds of conditioning. Neither the use of different filter brands, nor the use of different filter materials in a sample batch are encouraged as different membrane materials or brands could potentially elute varying impurities. The Royal Society of Chemistry 2019-10-08 /pmc/articles/PMC9116114/ /pubmed/35702663 http://dx.doi.org/10.1039/c9ra06198c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Puah, Perng Yang
Herng Lee, Dexter Jiunn
Mak, Ken Hing
Ang, Hui Jun
Chen, Hsing-Chang
Moh, Pak Yan
Fong, Siat Yee
Ling, Yee Soon
Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
title Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
title_full Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
title_fullStr Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
title_full_unstemmed Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
title_short Extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
title_sort extractable impurities from fluoropolymer-based membrane filters – interference in high-throughput, untargeted analysis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116114/
https://www.ncbi.nlm.nih.gov/pubmed/35702663
http://dx.doi.org/10.1039/c9ra06198c
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