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Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress

The mechanism of resistance of plants to cold temperatures is very complicated, and the molecular mechanism and related gene network in pepper are largely unknown. Here, during cold treatment, we used cluster analysis (k-means) to classify all expressed genes into 15 clusters, 3,680 and 2,405 differ...

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Autores principales: Tang, Bingqian, Xie, Lingling, Yang, Huiping, Li, Xiumin, Chen, Ying, Zou, Xuexiao, Liu, Feng, Dai, Xiongze
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116226/
https://www.ncbi.nlm.nih.gov/pubmed/35599873
http://dx.doi.org/10.3389/fpls.2022.852511
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author Tang, Bingqian
Xie, Lingling
Yang, Huiping
Li, Xiumin
Chen, Ying
Zou, Xuexiao
Liu, Feng
Dai, Xiongze
author_facet Tang, Bingqian
Xie, Lingling
Yang, Huiping
Li, Xiumin
Chen, Ying
Zou, Xuexiao
Liu, Feng
Dai, Xiongze
author_sort Tang, Bingqian
collection PubMed
description The mechanism of resistance of plants to cold temperatures is very complicated, and the molecular mechanism and related gene network in pepper are largely unknown. Here, during cold treatment, we used cluster analysis (k-means) to classify all expressed genes into 15 clusters, 3,680 and 2,405 differentially expressed genes (DEGs) were observed in the leaf and root, respectively. The DEGs associated with certain important basic metabolic processes, oxidoreductase activity, and overall membrane compositions were most significantly enriched. In addition, based on the homologous sequence alignment of Arabidopsis genes, we identified 14 positive and negative regulators of the ICE-CBF-COR module in pepper, including CBF and ICE, and compared their levels in different data sets. The correlation matrix constructed based on the expression patterns of whole pepper genes in leaves and roots after exposure to cold stress showed the correlation between 14 ICE-CBF-COR signaling module genes, and provided insight into the relationship between these genes in pepper. These findings not only provide valuable resources for research on cold tolerance, but also lay the foundation for the genetic modification of cold stress regulators, which would help us achieve improved crop tolerance. To our knowledge, this is the first study to demonstrate the relationship between positive and negative regulators related to the ICE-CBF-COR module, which is of great significance to the study of low-temperature adaptive mechanisms in plants.
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spelling pubmed-91162262022-05-19 Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress Tang, Bingqian Xie, Lingling Yang, Huiping Li, Xiumin Chen, Ying Zou, Xuexiao Liu, Feng Dai, Xiongze Front Plant Sci Plant Science The mechanism of resistance of plants to cold temperatures is very complicated, and the molecular mechanism and related gene network in pepper are largely unknown. Here, during cold treatment, we used cluster analysis (k-means) to classify all expressed genes into 15 clusters, 3,680 and 2,405 differentially expressed genes (DEGs) were observed in the leaf and root, respectively. The DEGs associated with certain important basic metabolic processes, oxidoreductase activity, and overall membrane compositions were most significantly enriched. In addition, based on the homologous sequence alignment of Arabidopsis genes, we identified 14 positive and negative regulators of the ICE-CBF-COR module in pepper, including CBF and ICE, and compared their levels in different data sets. The correlation matrix constructed based on the expression patterns of whole pepper genes in leaves and roots after exposure to cold stress showed the correlation between 14 ICE-CBF-COR signaling module genes, and provided insight into the relationship between these genes in pepper. These findings not only provide valuable resources for research on cold tolerance, but also lay the foundation for the genetic modification of cold stress regulators, which would help us achieve improved crop tolerance. To our knowledge, this is the first study to demonstrate the relationship between positive and negative regulators related to the ICE-CBF-COR module, which is of great significance to the study of low-temperature adaptive mechanisms in plants. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9116226/ /pubmed/35599873 http://dx.doi.org/10.3389/fpls.2022.852511 Text en Copyright © 2022 Tang, Xie, Yang, Li, Chen, Zou, Liu and Dai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tang, Bingqian
Xie, Lingling
Yang, Huiping
Li, Xiumin
Chen, Ying
Zou, Xuexiao
Liu, Feng
Dai, Xiongze
Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress
title Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress
title_full Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress
title_fullStr Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress
title_full_unstemmed Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress
title_short Analysis of the Expression and Function of Key Genes in Pepper Under Low-Temperature Stress
title_sort analysis of the expression and function of key genes in pepper under low-temperature stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116226/
https://www.ncbi.nlm.nih.gov/pubmed/35599873
http://dx.doi.org/10.3389/fpls.2022.852511
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