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Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma

Mitochondrial fission regulator 2 (MTFR2) belongs to the MTFR1 family, which plays a crucial role in regulating oxidative phosphorylation. Recent studies indicate that it also participates in cancer carcinogenesis and development; however, the clinical significance of MTFR2 in lung adenocarcinoma ha...

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Autores principales: Lian, Zengzhi, Pang, Pei, Zhu, Yan, Du, Wenwen, Zhou, Jintao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117628/
https://www.ncbi.nlm.nih.gov/pubmed/35600359
http://dx.doi.org/10.3389/fonc.2022.832517
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author Lian, Zengzhi
Pang, Pei
Zhu, Yan
Du, Wenwen
Zhou, Jintao
author_facet Lian, Zengzhi
Pang, Pei
Zhu, Yan
Du, Wenwen
Zhou, Jintao
author_sort Lian, Zengzhi
collection PubMed
description Mitochondrial fission regulator 2 (MTFR2) belongs to the MTFR1 family, which plays a crucial role in regulating oxidative phosphorylation. Recent studies indicate that it also participates in cancer carcinogenesis and development; however, the clinical significance of MTFR2 in lung adenocarcinoma has not been fully confirmed. Our current study investigated the relationships between clinical characteristics and MTFR2 expression based on The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GSE31210) dataset, and clinical histopathological sample cohort. In addition, Kaplan–Meier and Cox regression analyses were additionally performed to evaluate the association between MTFR2 expression and patient survival. Gene set enrichment analysis (GESA) was conducted to spot possible pathways associated with MTFR2. Moreover, a single-sample GESA (ssGESA) was performed to evaluate the association between MTFR2 expression and immune cell infiltration. Cell colony formation assay, CCK-8 assay, cell cycle assay, and transwell assay were performed to verify the cell proliferation, migration, and invasion abilities after interfering with MTFR2 in lung cancer cells. Western blot assay was applied to identify the underlying protein levels. The results indicated that the elevated MTFR2 expression in lung adenocarcinoma samples correlated with T stage (P < 0.001), N stage (P = 0.005), M stage (P = 0.015), pathological stage (P = 0.002), and TP53 status (P < 0.001). Patients with a higher MTFR2 expression correlated with poorer overall survival (P < 0.01) and progression-free survival (P = 0.002). Knockdown of MTFR2 inhibited cell proliferation, migration, and invasion via AKT-cyclin D1 signaling and EMT pathways. Moreover, MTFR2 expression significantly positively correlated with Th2 cells (P < 0.001). Taken together, MTFR2 could serve as a novel prognostic indicator and therapeutic target for lung adenocarcinoma.
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spelling pubmed-91176282022-05-20 Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma Lian, Zengzhi Pang, Pei Zhu, Yan Du, Wenwen Zhou, Jintao Front Oncol Oncology Mitochondrial fission regulator 2 (MTFR2) belongs to the MTFR1 family, which plays a crucial role in regulating oxidative phosphorylation. Recent studies indicate that it also participates in cancer carcinogenesis and development; however, the clinical significance of MTFR2 in lung adenocarcinoma has not been fully confirmed. Our current study investigated the relationships between clinical characteristics and MTFR2 expression based on The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GSE31210) dataset, and clinical histopathological sample cohort. In addition, Kaplan–Meier and Cox regression analyses were additionally performed to evaluate the association between MTFR2 expression and patient survival. Gene set enrichment analysis (GESA) was conducted to spot possible pathways associated with MTFR2. Moreover, a single-sample GESA (ssGESA) was performed to evaluate the association between MTFR2 expression and immune cell infiltration. Cell colony formation assay, CCK-8 assay, cell cycle assay, and transwell assay were performed to verify the cell proliferation, migration, and invasion abilities after interfering with MTFR2 in lung cancer cells. Western blot assay was applied to identify the underlying protein levels. The results indicated that the elevated MTFR2 expression in lung adenocarcinoma samples correlated with T stage (P < 0.001), N stage (P = 0.005), M stage (P = 0.015), pathological stage (P = 0.002), and TP53 status (P < 0.001). Patients with a higher MTFR2 expression correlated with poorer overall survival (P < 0.01) and progression-free survival (P = 0.002). Knockdown of MTFR2 inhibited cell proliferation, migration, and invasion via AKT-cyclin D1 signaling and EMT pathways. Moreover, MTFR2 expression significantly positively correlated with Th2 cells (P < 0.001). Taken together, MTFR2 could serve as a novel prognostic indicator and therapeutic target for lung adenocarcinoma. Frontiers Media S.A. 2022-05-05 /pmc/articles/PMC9117628/ /pubmed/35600359 http://dx.doi.org/10.3389/fonc.2022.832517 Text en Copyright © 2022 Lian, Pang, Zhu, Du and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Lian, Zengzhi
Pang, Pei
Zhu, Yan
Du, Wenwen
Zhou, Jintao
Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma
title Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma
title_full Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma
title_fullStr Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma
title_full_unstemmed Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma
title_short Prognostic Value and Potential Mechanism of MTFR2 in Lung Adenocarcinoma
title_sort prognostic value and potential mechanism of mtfr2 in lung adenocarcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117628/
https://www.ncbi.nlm.nih.gov/pubmed/35600359
http://dx.doi.org/10.3389/fonc.2022.832517
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