A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced mice

Highly enriched germinal center (GC) B cell populations are essential for studying humoral immunity. Current MACS protocols that isolate untouched GC B cells require GC induction and typically require further FACS purification with direct antibody labeling to achieve sufficiently high purities. We p...

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Detalles Bibliográficos
Autores principales: Desikan, Shreya A., Chavan, Sailee, Ou, Peiqi, Roman, Christopher A.J., Huan, Chongmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117920/
https://www.ncbi.nlm.nih.gov/pubmed/35600926
http://dx.doi.org/10.1016/j.xpro.2022.101388

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