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Primary culture of tracheal aspirate-derived human airway basal stem cells

Patient-specific airway basal stem cells (BSCs) can be derived from tracheal aspirate (TA) samples from intubated patients, thus providing an invaluable lung stem cell derivation method that bypasses the need for lung tissue. The primary culture of BSCs provides the ideal model to study the function...

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Detalles Bibliográficos
Autores principales: Amonkar, Gaurang M., Wagner, Richard, Bankoti, Kamakshi, Shui, Jessica E., Ai, Xingbin, Lerou, Paul H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117922/
https://www.ncbi.nlm.nih.gov/pubmed/35600918
http://dx.doi.org/10.1016/j.xpro.2022.101390
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author Amonkar, Gaurang M.
Wagner, Richard
Bankoti, Kamakshi
Shui, Jessica E.
Ai, Xingbin
Lerou, Paul H.
author_facet Amonkar, Gaurang M.
Wagner, Richard
Bankoti, Kamakshi
Shui, Jessica E.
Ai, Xingbin
Lerou, Paul H.
author_sort Amonkar, Gaurang M.
collection PubMed
description Patient-specific airway basal stem cells (BSCs) can be derived from tracheal aspirate (TA) samples from intubated patients, thus providing an invaluable lung stem cell derivation method that bypasses the need for lung tissue. The primary culture of BSCs provides the ideal model to study the function and differentiation of the conducting lung epithelium. This protocol outlines the specific steps for isolation, culture maintenance, passaging, freezing, thawing, differentiation, and immunofluorescence characterization of human TA-derived airway BSCs. For complete details on the use and execution of this protocol, please refer to Lu et al. (2021).
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spelling pubmed-91179222022-05-20 Primary culture of tracheal aspirate-derived human airway basal stem cells Amonkar, Gaurang M. Wagner, Richard Bankoti, Kamakshi Shui, Jessica E. Ai, Xingbin Lerou, Paul H. STAR Protoc Protocol Patient-specific airway basal stem cells (BSCs) can be derived from tracheal aspirate (TA) samples from intubated patients, thus providing an invaluable lung stem cell derivation method that bypasses the need for lung tissue. The primary culture of BSCs provides the ideal model to study the function and differentiation of the conducting lung epithelium. This protocol outlines the specific steps for isolation, culture maintenance, passaging, freezing, thawing, differentiation, and immunofluorescence characterization of human TA-derived airway BSCs. For complete details on the use and execution of this protocol, please refer to Lu et al. (2021). Elsevier 2022-05-13 /pmc/articles/PMC9117922/ /pubmed/35600918 http://dx.doi.org/10.1016/j.xpro.2022.101390 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Amonkar, Gaurang M.
Wagner, Richard
Bankoti, Kamakshi
Shui, Jessica E.
Ai, Xingbin
Lerou, Paul H.
Primary culture of tracheal aspirate-derived human airway basal stem cells
title Primary culture of tracheal aspirate-derived human airway basal stem cells
title_full Primary culture of tracheal aspirate-derived human airway basal stem cells
title_fullStr Primary culture of tracheal aspirate-derived human airway basal stem cells
title_full_unstemmed Primary culture of tracheal aspirate-derived human airway basal stem cells
title_short Primary culture of tracheal aspirate-derived human airway basal stem cells
title_sort primary culture of tracheal aspirate-derived human airway basal stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117922/
https://www.ncbi.nlm.nih.gov/pubmed/35600918
http://dx.doi.org/10.1016/j.xpro.2022.101390
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