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In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture

Nuclear Matrix (NuMat) is a biochemically defined entity that provides us with a snapshot of the features of the nuclear architecture. Here, we present a protocol to isolate and visualize NuMat in situ in the intact embryo or tissues of Drosophila melanogaster and its applications. We remove the chr...

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Detalles Bibliográficos
Autores principales: Pathak, Rashmi U., Bihani, Ashish, Sureka, Rahul, Mishra, Rakesh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117923/
https://www.ncbi.nlm.nih.gov/pubmed/35600936
http://dx.doi.org/10.1016/j.xpro.2022.101394