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Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse

Spermatogenesis generates heterologous cell populations which, if not distinguished clearly, often hinder mechanistic and etiological studies. Here, we present a protocol to identify and isolate populations of mouse spermatogenic cells, including spermatogonial stem cells (SSCs), spermatocytes, and...

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Detalles Bibliográficos
Autores principales: Zou, Qianxing, Yang, Lele, Qi, Huayu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117932/
https://www.ncbi.nlm.nih.gov/pubmed/35600921
http://dx.doi.org/10.1016/j.xpro.2022.101398
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author Zou, Qianxing
Yang, Lele
Qi, Huayu
author_facet Zou, Qianxing
Yang, Lele
Qi, Huayu
author_sort Zou, Qianxing
collection PubMed
description Spermatogenesis generates heterologous cell populations which, if not distinguished clearly, often hinder mechanistic and etiological studies. Here, we present a protocol to identify and isolate populations of mouse spermatogenic cells, including spermatogonial stem cells (SSCs), spermatocytes, and haploid spermatids. We also describe absolute quantification of mRNA copy numbers in SSCs. The isolated cells can be used for analyzing nascent protein synthesis and protein degradation, two main events that maintain cellular proteostasis important for healthy and long-term production of male gametes. For complete details on the use and execution of this protocol, please refer to Zou et al. (2021).
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spelling pubmed-91179322022-05-20 Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse Zou, Qianxing Yang, Lele Qi, Huayu STAR Protoc Protocol Spermatogenesis generates heterologous cell populations which, if not distinguished clearly, often hinder mechanistic and etiological studies. Here, we present a protocol to identify and isolate populations of mouse spermatogenic cells, including spermatogonial stem cells (SSCs), spermatocytes, and haploid spermatids. We also describe absolute quantification of mRNA copy numbers in SSCs. The isolated cells can be used for analyzing nascent protein synthesis and protein degradation, two main events that maintain cellular proteostasis important for healthy and long-term production of male gametes. For complete details on the use and execution of this protocol, please refer to Zou et al. (2021). Elsevier 2022-05-14 /pmc/articles/PMC9117932/ /pubmed/35600921 http://dx.doi.org/10.1016/j.xpro.2022.101398 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zou, Qianxing
Yang, Lele
Qi, Huayu
Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
title Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
title_full Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
title_fullStr Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
title_full_unstemmed Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
title_short Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
title_sort protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117932/
https://www.ncbi.nlm.nih.gov/pubmed/35600921
http://dx.doi.org/10.1016/j.xpro.2022.101398
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