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Mounting Drosophila pupae for laser ablation and live imaging of the dorsal thorax
This protocol describes the preparation of Drosophilamelanogaster pupae for laser ablation and live imaging of the notum (dorsal thorax). Because the pupa is stationary, it can be continuously live imaged for multiple days if desired, making it ideal for studying wound signaling and repair, from bef...
Autores principales: | O’Connor, James T., Shannon, Erica K., Hutson, M. Shane, Page-McCaw, Andrea |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117934/ https://www.ncbi.nlm.nih.gov/pubmed/35600923 http://dx.doi.org/10.1016/j.xpro.2022.101396 |
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