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Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis

On September 21, 2019, the Shenzhen and Dongguan Centers for Disease Control and Prevention received notification of a large cluster of suspected gastroenteritis involving primarily children who sought medical care at hospitals throughout two adjacent cities in China, Shenzhen, and Dongguan. A joint...

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Autores principales: Jiang, Min, Yang, Chao, Kwan, Patrick S. L., Zhang, Liping, Fan, Hang, Jin, Yujuan, Sun, Lifang, Chen, Hongyu, Li, Baisheng, Chen, Qiuxia, Wu, Yarong, Guo, Yan, Shi, Yuanguo, Liao, Min, Shi, Xiaolu, Liu, Jianping, Jiang, Lijuan, Cai, Rui, Deng, Yinhua, Sun, Qun, Yang, Ruifu, Zhang, Qiaoli, Cui, Yujun, Hu, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117964/
https://www.ncbi.nlm.nih.gov/pubmed/35602015
http://dx.doi.org/10.3389/fmicb.2022.772489
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author Jiang, Min
Yang, Chao
Kwan, Patrick S. L.
Zhang, Liping
Fan, Hang
Jin, Yujuan
Sun, Lifang
Chen, Hongyu
Li, Baisheng
Chen, Qiuxia
Wu, Yarong
Guo, Yan
Shi, Yuanguo
Liao, Min
Shi, Xiaolu
Liu, Jianping
Jiang, Lijuan
Cai, Rui
Deng, Yinhua
Sun, Qun
Yang, Ruifu
Zhang, Qiaoli
Cui, Yujun
Hu, Qinghua
author_facet Jiang, Min
Yang, Chao
Kwan, Patrick S. L.
Zhang, Liping
Fan, Hang
Jin, Yujuan
Sun, Lifang
Chen, Hongyu
Li, Baisheng
Chen, Qiuxia
Wu, Yarong
Guo, Yan
Shi, Yuanguo
Liao, Min
Shi, Xiaolu
Liu, Jianping
Jiang, Lijuan
Cai, Rui
Deng, Yinhua
Sun, Qun
Yang, Ruifu
Zhang, Qiaoli
Cui, Yujun
Hu, Qinghua
author_sort Jiang, Min
collection PubMed
description On September 21, 2019, the Shenzhen and Dongguan Centers for Disease Control and Prevention received notification of a large cluster of suspected gastroenteritis involving primarily children who sought medical care at hospitals throughout two adjacent cities in China, Shenzhen, and Dongguan. A joint outbreak response was promptly initiated across jurisdictions in a concerted effort between clinical microbiologists, epidemiologists, and public health scientists. Concurrently, multiplex PCRs were used for rapid laboratory diagnosis of suspected cases; epidemiological investigations were conducted to identify the outbreak source, complemented by near real-time multicenter whole-genome analyses completed within 34 h. Epidemiological evidence indicated that all patients had consumed egg sandwiches served on September 20 as snacks to children and staff at a nursery in Dongguan, located near Shenzhen. Salmonella Enteritidis was isolated from case-patients, food handlers, kitchenware, and sandwiches with kitchen-made mayonnaise. Whole-genome single-nucleotide polymorphism (SNP)-based phylogenetic analysis demonstrated a well-supported cluster with pairwise distances of ≤1 SNP between genomes for outbreak-associated isolates, providing the definitive link between all samples. In comparison with historical isolates from the same geographical region, the minimum pairwise distance was >14 SNPs, suggesting a non-local outbreak source. Genomic source tracing revealed the possible transmission dynamics of a S. Enteritidis clone throughout a multi-provincial egg distribution network. The efficiency and scale with which multidisciplinary and integrated approaches were coordinated in this foodborne disease outbreak response was unprecedented in China, leading to the timely intervention of a large cross-jurisdiction Salmonella outbreak.
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spelling pubmed-91179642022-05-20 Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis Jiang, Min Yang, Chao Kwan, Patrick S. L. Zhang, Liping Fan, Hang Jin, Yujuan Sun, Lifang Chen, Hongyu Li, Baisheng Chen, Qiuxia Wu, Yarong Guo, Yan Shi, Yuanguo Liao, Min Shi, Xiaolu Liu, Jianping Jiang, Lijuan Cai, Rui Deng, Yinhua Sun, Qun Yang, Ruifu Zhang, Qiaoli Cui, Yujun Hu, Qinghua Front Microbiol Microbiology On September 21, 2019, the Shenzhen and Dongguan Centers for Disease Control and Prevention received notification of a large cluster of suspected gastroenteritis involving primarily children who sought medical care at hospitals throughout two adjacent cities in China, Shenzhen, and Dongguan. A joint outbreak response was promptly initiated across jurisdictions in a concerted effort between clinical microbiologists, epidemiologists, and public health scientists. Concurrently, multiplex PCRs were used for rapid laboratory diagnosis of suspected cases; epidemiological investigations were conducted to identify the outbreak source, complemented by near real-time multicenter whole-genome analyses completed within 34 h. Epidemiological evidence indicated that all patients had consumed egg sandwiches served on September 20 as snacks to children and staff at a nursery in Dongguan, located near Shenzhen. Salmonella Enteritidis was isolated from case-patients, food handlers, kitchenware, and sandwiches with kitchen-made mayonnaise. Whole-genome single-nucleotide polymorphism (SNP)-based phylogenetic analysis demonstrated a well-supported cluster with pairwise distances of ≤1 SNP between genomes for outbreak-associated isolates, providing the definitive link between all samples. In comparison with historical isolates from the same geographical region, the minimum pairwise distance was >14 SNPs, suggesting a non-local outbreak source. Genomic source tracing revealed the possible transmission dynamics of a S. Enteritidis clone throughout a multi-provincial egg distribution network. The efficiency and scale with which multidisciplinary and integrated approaches were coordinated in this foodborne disease outbreak response was unprecedented in China, leading to the timely intervention of a large cross-jurisdiction Salmonella outbreak. Frontiers Media S.A. 2022-05-04 /pmc/articles/PMC9117964/ /pubmed/35602015 http://dx.doi.org/10.3389/fmicb.2022.772489 Text en Copyright © 2022 Jiang, Yang, Kwan, Zhang, Fan, Jin, Sun, Chen, Li, Chen, Wu, Guo, Shi, Liao, Shi, Liu, Jiang, Cai, Deng, Sun, Yang, Zhang, Cui and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Jiang, Min
Yang, Chao
Kwan, Patrick S. L.
Zhang, Liping
Fan, Hang
Jin, Yujuan
Sun, Lifang
Chen, Hongyu
Li, Baisheng
Chen, Qiuxia
Wu, Yarong
Guo, Yan
Shi, Yuanguo
Liao, Min
Shi, Xiaolu
Liu, Jianping
Jiang, Lijuan
Cai, Rui
Deng, Yinhua
Sun, Qun
Yang, Ruifu
Zhang, Qiaoli
Cui, Yujun
Hu, Qinghua
Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis
title Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis
title_full Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis
title_fullStr Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis
title_full_unstemmed Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis
title_short Rapid Multilateral and Integrated Public Health Response to a Cross-City Outbreak of Salmonella Enteritidis Infections Combining Analytical, Molecular, and Genomic Epidemiological Analysis
title_sort rapid multilateral and integrated public health response to a cross-city outbreak of salmonella enteritidis infections combining analytical, molecular, and genomic epidemiological analysis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117964/
https://www.ncbi.nlm.nih.gov/pubmed/35602015
http://dx.doi.org/10.3389/fmicb.2022.772489
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