Enhanced Catalytic Activity of a New Nanobiocatalytic System Formed by the Adsorption of Cytochrome c on Pluronic Triblock Copolymer Stabilized MoS(2) Nanosheets

[Image: see text] The formation of nanobiohybrids through the immobilization of enzymes on functional nanomaterials has opened up exciting research opportunities at the nanobiointerfaces. These systems hold great promise for a wide range of applications in biosensing, biocatalytic, and biomedical fields. Here, we report the formation of a hybrid nanobiocatalytic system through the adsorption of cytochrome c (Cyt c) on pluronic triblock copolymer, P123 (PEO-b-PPO-b-PEO), stabilized MoS(2) nanosheets. The use of pluronic polymer has helped not only to greatly stabilize the exfoliated MoS(2) nanosheets but also to allow easy adsorption of Cyt c on the nanosheets without major structural changes due to its excellent biocompatibility and soft protein-binding property. By comparing the catalytic activity of the Cyt c–MoS(2) nanobiohybrid with that of the free Cyt c and as-prepared MoS(2) nanosheets, we have demonstrated the active role of the nanobiointeractions in enhancing the catalytic activity of the hybrid. Slight structural perturbation at the active site of the Cyt c upon adsorption on MoS(2) has primarily facilitated the peroxidase activity of the Cyt c. As the MoS(2) nanosheets and the native Cyt c individually exhibit weaker intrinsic peroxidase activities, their mutual modulation at the nanobiointerface has made the Cyt c–MoS(2) a novel nanobiocatalyst with superior activity.