Cargando…

Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces

BACKGROUND: The current investigation aims to green synthesized the zinc nanoparticles (ZnNPs) using Lavandula angustifolia extract by microwave technique and its protoscolicidal effects alone and combined with albendazole against hydatid cyst protoscoleces. METHODS: Different concentrations of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Shakibaie, Mojtaba, Khalaf, Amal Khudair, Rashidipour, Marziyeh, Mahmoudvand, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118496/
https://www.ncbi.nlm.nih.gov/pubmed/35600184
http://dx.doi.org/10.1016/j.amsu.2022.103746
_version_ 1784710508887146496
author Shakibaie, Mojtaba
Khalaf, Amal Khudair
Rashidipour, Marziyeh
Mahmoudvand, Hossein
author_facet Shakibaie, Mojtaba
Khalaf, Amal Khudair
Rashidipour, Marziyeh
Mahmoudvand, Hossein
author_sort Shakibaie, Mojtaba
collection PubMed
description BACKGROUND: The current investigation aims to green synthesized the zinc nanoparticles (ZnNPs) using Lavandula angustifolia extract by microwave technique and its protoscolicidal effects alone and combined with albendazole against hydatid cyst protoscoleces. METHODS: Different concentrations of the ZnNPs (50, 100, and 200 μg/ml) alone and combined with albendazole (ALZ, 100 μg/ml) were treated with hydatid cyst protoscoleces obtained from liver of infected sheep for 5–60 min in vitro and ex vivo. Eosin exclusion examination was used to assess the viability of protoscoleces. The induction of apoptosis in hydatid cyst protoscoleces was assessed by measurement of the Caspase-3 activity of protoscoleces treated with various concentrations of ZnNPs. RESULTS: The size of green synthesized ZnNPs was ranged from 30 to 80 nm, most of these nanoparticles were between 50 and 60 nm in size. In vitro, the highest scolicidal effect of ZnNPs was observed at the concentration of 200 μg/ml, where it killed 81.6% of protoscolices. While the combination of these nanoparticles with ALZ, especially at the concentration of 200 μg/ml, completely killed the protoscolices after 10 min’ exposure. However, compared to in vitro assay, the drugs tested took longer to show their protoscolicidal effect. CONCLUSION: Based on the obtained results, ZnNPs particularly in combination with albendazole displayed the potent protoscolicidal in vitro and ex vivo as an intraperitoneal model of administration of agents to hydatid cyst treatment; nevertheless, additional investigations are mandatory to evaluate the efficacy and safety Zn NPs as a favorable protoscolicidal agent in clinical setting.
format Online
Article
Text
id pubmed-9118496
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-91184962022-05-20 Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces Shakibaie, Mojtaba Khalaf, Amal Khudair Rashidipour, Marziyeh Mahmoudvand, Hossein Ann Med Surg (Lond) Cohort Study BACKGROUND: The current investigation aims to green synthesized the zinc nanoparticles (ZnNPs) using Lavandula angustifolia extract by microwave technique and its protoscolicidal effects alone and combined with albendazole against hydatid cyst protoscoleces. METHODS: Different concentrations of the ZnNPs (50, 100, and 200 μg/ml) alone and combined with albendazole (ALZ, 100 μg/ml) were treated with hydatid cyst protoscoleces obtained from liver of infected sheep for 5–60 min in vitro and ex vivo. Eosin exclusion examination was used to assess the viability of protoscoleces. The induction of apoptosis in hydatid cyst protoscoleces was assessed by measurement of the Caspase-3 activity of protoscoleces treated with various concentrations of ZnNPs. RESULTS: The size of green synthesized ZnNPs was ranged from 30 to 80 nm, most of these nanoparticles were between 50 and 60 nm in size. In vitro, the highest scolicidal effect of ZnNPs was observed at the concentration of 200 μg/ml, where it killed 81.6% of protoscolices. While the combination of these nanoparticles with ALZ, especially at the concentration of 200 μg/ml, completely killed the protoscolices after 10 min’ exposure. However, compared to in vitro assay, the drugs tested took longer to show their protoscolicidal effect. CONCLUSION: Based on the obtained results, ZnNPs particularly in combination with albendazole displayed the potent protoscolicidal in vitro and ex vivo as an intraperitoneal model of administration of agents to hydatid cyst treatment; nevertheless, additional investigations are mandatory to evaluate the efficacy and safety Zn NPs as a favorable protoscolicidal agent in clinical setting. Elsevier 2022-05-11 /pmc/articles/PMC9118496/ /pubmed/35600184 http://dx.doi.org/10.1016/j.amsu.2022.103746 Text en © 2022 Published by Elsevier Ltd on behalf of IJS Publishing Group Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Cohort Study
Shakibaie, Mojtaba
Khalaf, Amal Khudair
Rashidipour, Marziyeh
Mahmoudvand, Hossein
Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
title Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
title_full Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
title_fullStr Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
title_full_unstemmed Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
title_short Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
title_sort effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces
topic Cohort Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118496/
https://www.ncbi.nlm.nih.gov/pubmed/35600184
http://dx.doi.org/10.1016/j.amsu.2022.103746
work_keys_str_mv AT shakibaiemojtaba effectsofgreensynthesizedzincnanoparticlesaloneandalongwithalbendazoleagainsthydatidcystprotoscoleces
AT khalafamalkhudair effectsofgreensynthesizedzincnanoparticlesaloneandalongwithalbendazoleagainsthydatidcystprotoscoleces
AT rashidipourmarziyeh effectsofgreensynthesizedzincnanoparticlesaloneandalongwithalbendazoleagainsthydatidcystprotoscoleces
AT mahmoudvandhossein effectsofgreensynthesizedzincnanoparticlesaloneandalongwithalbendazoleagainsthydatidcystprotoscoleces