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Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia

BACKGROUND: Antimicrobial resistance (AMR) is a global public health threat and the use of antibiotics growth promoters in food animals has been implicated as a potential contributing factor in the emergence and spread of AMR. This study was conducted to investigate colistin and carbapenem resistanc...

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Autores principales: Aklilu, Erkihun, Harun, Azian, Singh, Kirnpal Kaur Banga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118571/
https://www.ncbi.nlm.nih.gov/pubmed/35590358
http://dx.doi.org/10.1186/s12917-022-03292-7
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author Aklilu, Erkihun
Harun, Azian
Singh, Kirnpal Kaur Banga
author_facet Aklilu, Erkihun
Harun, Azian
Singh, Kirnpal Kaur Banga
author_sort Aklilu, Erkihun
collection PubMed
description BACKGROUND: Antimicrobial resistance (AMR) is a global public health threat and the use of antibiotics growth promoters in food animals has been implicated as a potential contributing factor in the emergence and spread of AMR. This study was conducted to investigate colistin and carbapenem resistance and extended spectrum beta-lactamase producing E. coli from live broiler chicken and chicken meat in Kelantan, Malaysia. RESULTS: Among the E. coli isolates, 37.5% (27/72 were positive for at least one of the resistance genes and one isolate was positive for mcr-1, bla(TEM-52), bla(NDM) and bla(OXA-48) whereas 4.17% (3/72) and 2.78% (2/72) were positive for mcr-1, bla(TEM-52) and bla(OXA-48), and mcr-1, bla(TEM-52) and bla(IMP). Multilocus sequence typing (MLST) results revealed the presence of widespread E. coli strains belonging to the sequence types ST410 and ST155 and other extra-intestinal E. coli (ExPEC) strains. Phylogroup A made up the majority 51.85% (14/27) followed by phylogroup B1 22.22% (6/27). CONCLUSIONS: The findings imply the potential threats of colistin, extended-spectrum beta-lactamase producing and carbapenem resistant E. coli in food animals to the public health and underscores the need for judicious use of antibiotics in animal production and good hygiene practices to curb the rising risks of AMR.
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spelling pubmed-91185712022-05-20 Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia Aklilu, Erkihun Harun, Azian Singh, Kirnpal Kaur Banga BMC Vet Res Research BACKGROUND: Antimicrobial resistance (AMR) is a global public health threat and the use of antibiotics growth promoters in food animals has been implicated as a potential contributing factor in the emergence and spread of AMR. This study was conducted to investigate colistin and carbapenem resistance and extended spectrum beta-lactamase producing E. coli from live broiler chicken and chicken meat in Kelantan, Malaysia. RESULTS: Among the E. coli isolates, 37.5% (27/72 were positive for at least one of the resistance genes and one isolate was positive for mcr-1, bla(TEM-52), bla(NDM) and bla(OXA-48) whereas 4.17% (3/72) and 2.78% (2/72) were positive for mcr-1, bla(TEM-52) and bla(OXA-48), and mcr-1, bla(TEM-52) and bla(IMP). Multilocus sequence typing (MLST) results revealed the presence of widespread E. coli strains belonging to the sequence types ST410 and ST155 and other extra-intestinal E. coli (ExPEC) strains. Phylogroup A made up the majority 51.85% (14/27) followed by phylogroup B1 22.22% (6/27). CONCLUSIONS: The findings imply the potential threats of colistin, extended-spectrum beta-lactamase producing and carbapenem resistant E. coli in food animals to the public health and underscores the need for judicious use of antibiotics in animal production and good hygiene practices to curb the rising risks of AMR. BioMed Central 2022-05-19 /pmc/articles/PMC9118571/ /pubmed/35590358 http://dx.doi.org/10.1186/s12917-022-03292-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Aklilu, Erkihun
Harun, Azian
Singh, Kirnpal Kaur Banga
Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia
title Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia
title_full Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia
title_fullStr Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia
title_full_unstemmed Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia
title_short Molecular characterization of bla(NDM), bla(OXA-48), mcr-1 and bla(TEM-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing Escherichia coli in chicken in Malaysia
title_sort molecular characterization of bla(ndm), bla(oxa-48), mcr-1 and bla(tem-52) positive and concurrently carbapenem and colistin resistant and extended spectrum beta-lactamase producing escherichia coli in chicken in malaysia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118571/
https://www.ncbi.nlm.nih.gov/pubmed/35590358
http://dx.doi.org/10.1186/s12917-022-03292-7
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