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Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia

OBJECTIVE: To analyze the results of the preimplantation genetic testing for aneuploidy at the Instituto de Fertilidad Humana - Inser Bogotá, Colombia, from 2016 to 2020. METHODS: This study is an observational, retrospective, and correlative analysis of biopsies from 319 embryos (from 54 patients)...

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Autores principales: Idárraga, Germán David Ospina, Suárez, Iván Darío Montes, Osorio, Katherine Gisell Hernández, Corredor, Diana Milena Diaz, Redondo, Johanna Carolina Páez, Yepes, Ricardo Garcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118953/
https://www.ncbi.nlm.nih.gov/pubmed/34812601
http://dx.doi.org/10.5935/1518-0557.20210085
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author Idárraga, Germán David Ospina
Suárez, Iván Darío Montes
Osorio, Katherine Gisell Hernández
Corredor, Diana Milena Diaz
Redondo, Johanna Carolina Páez
Yepes, Ricardo Garcia
author_facet Idárraga, Germán David Ospina
Suárez, Iván Darío Montes
Osorio, Katherine Gisell Hernández
Corredor, Diana Milena Diaz
Redondo, Johanna Carolina Páez
Yepes, Ricardo Garcia
author_sort Idárraga, Germán David Ospina
collection PubMed
description OBJECTIVE: To analyze the results of the preimplantation genetic testing for aneuploidy at the Instituto de Fertilidad Humana - Inser Bogotá, Colombia, from 2016 to 2020. METHODS: This study is an observational, retrospective, and correlative analysis of biopsies from 319 embryos (from 54 patients) submitted to preimplantation genetic testing for aneuploidy by different molecular techniques. RESULTS: Of the 54 patients included in the study, 42 provided their own oocytes, and 12 used donated oocytes. The main indication to perform the preimplantation genetic testing was advanced maternal age. We obtained 319 embryos: Ninety-one (28.5%) euploid, 197 (61.8%) aneuploid and 31 (9.7%) with no detectable DNA. The highest rate of aneuploid embryos was found in patients over 40 years (72.7%), and the euploidy rate in patients under 35 years was 37.1%. After the transfer of euploid embryos, the rates for implantation, ongoing pregnancy, live birth, and miscarriage were 40%, 50%, 40.6%, and 0%, respectively. Older maternal age correlated with higher numbers of aneuploid embryos and lower numbers of both euploid and 5-day embryos. CONCLUSIONS: There was a positive correlation between maternal age and aneuploidy rate. Complex chromosomal abnormalities were the most frequent aneuploidies, followed by mosaicism and double aneuploidies. The miscarriage rate after the transfer of euploid embryos was 0 %.
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spelling pubmed-91189532022-05-20 Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia Idárraga, Germán David Ospina Suárez, Iván Darío Montes Osorio, Katherine Gisell Hernández Corredor, Diana Milena Diaz Redondo, Johanna Carolina Páez Yepes, Ricardo Garcia JBRA Assist Reprod Original Article OBJECTIVE: To analyze the results of the preimplantation genetic testing for aneuploidy at the Instituto de Fertilidad Humana - Inser Bogotá, Colombia, from 2016 to 2020. METHODS: This study is an observational, retrospective, and correlative analysis of biopsies from 319 embryos (from 54 patients) submitted to preimplantation genetic testing for aneuploidy by different molecular techniques. RESULTS: Of the 54 patients included in the study, 42 provided their own oocytes, and 12 used donated oocytes. The main indication to perform the preimplantation genetic testing was advanced maternal age. We obtained 319 embryos: Ninety-one (28.5%) euploid, 197 (61.8%) aneuploid and 31 (9.7%) with no detectable DNA. The highest rate of aneuploid embryos was found in patients over 40 years (72.7%), and the euploidy rate in patients under 35 years was 37.1%. After the transfer of euploid embryos, the rates for implantation, ongoing pregnancy, live birth, and miscarriage were 40%, 50%, 40.6%, and 0%, respectively. Older maternal age correlated with higher numbers of aneuploid embryos and lower numbers of both euploid and 5-day embryos. CONCLUSIONS: There was a positive correlation between maternal age and aneuploidy rate. Complex chromosomal abnormalities were the most frequent aneuploidies, followed by mosaicism and double aneuploidies. The miscarriage rate after the transfer of euploid embryos was 0 %. Brazilian Society of Assisted Reproduction 2022 /pmc/articles/PMC9118953/ /pubmed/34812601 http://dx.doi.org/10.5935/1518-0557.20210085 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Idárraga, Germán David Ospina
Suárez, Iván Darío Montes
Osorio, Katherine Gisell Hernández
Corredor, Diana Milena Diaz
Redondo, Johanna Carolina Páez
Yepes, Ricardo Garcia
Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia
title Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia
title_full Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia
title_fullStr Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia
title_full_unstemmed Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia
title_short Results of Preimplantation Genetic Testing for Aneuploidy (PGT-A) in a Cohort of 319 Embryos: Experience in a Fertility Clinic in Colombia
title_sort results of preimplantation genetic testing for aneuploidy (pgt-a) in a cohort of 319 embryos: experience in a fertility clinic in colombia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118953/
https://www.ncbi.nlm.nih.gov/pubmed/34812601
http://dx.doi.org/10.5935/1518-0557.20210085
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