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A Protocol for the Cryopreservation of Human Intestinal Mucosal Biopsies Compatible With Single-Cell Transcriptomics and Ex Vivo Studies

The heterogeneity of the human intestinal epithelium has hindered the understanding of the pathophysiology of distinct specialized cell types on a single-cell basis in disease states. Described here is a workflow for the cryopreservation of endoscopically obtained human intestinal mucosal biopsies,...

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Detalles Bibliográficos
Autores principales:	McRae, Alison, Ricardo-Silgado, Maria Laura, Liu, Yuanhang, Calderon, Gerardo, Gonzalez-Izundegui, Daniel, Rohakhtar, Fariborz Rakhshan, Simon, Vernadette, Li, Ying, Acosta, Andres
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Physiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119022/ https://www.ncbi.nlm.nih.gov/pubmed/35600311 http://dx.doi.org/10.3389/fphys.2022.878389

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