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Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers

Genetic purity is a prerequisite for exploiting the potential of hybrids in cross-pollinated crops, such as sunflower. In this regard DNA-based study was conducted using 110 simple sequence repeat (SSR) markers to check the genetic purity of 23 parents and their 60 hybrids in sunflower. The polymorp...

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Autores principales: Ahmed, Hafiz Ghulam Muhu-Din, Rizwan, Muhammad, Naeem, Muhammad, Khan, Muhammad Ahsan, Baloch, Faheem Shehzad, Sun, Sangmi, Chung, Gyuhwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119457/
https://www.ncbi.nlm.nih.gov/pubmed/35588423
http://dx.doi.org/10.1371/journal.pone.0267383
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author Ahmed, Hafiz Ghulam Muhu-Din
Rizwan, Muhammad
Naeem, Muhammad
Khan, Muhammad Ahsan
Baloch, Faheem Shehzad
Sun, Sangmi
Chung, Gyuhwa
author_facet Ahmed, Hafiz Ghulam Muhu-Din
Rizwan, Muhammad
Naeem, Muhammad
Khan, Muhammad Ahsan
Baloch, Faheem Shehzad
Sun, Sangmi
Chung, Gyuhwa
author_sort Ahmed, Hafiz Ghulam Muhu-Din
collection PubMed
description Genetic purity is a prerequisite for exploiting the potential of hybrids in cross-pollinated crops, such as sunflower. In this regard DNA-based study was conducted using 110 simple sequence repeat (SSR) markers to check the genetic purity of 23 parents and their 60 hybrids in sunflower. The polymorphism was shown in 92 markers with value 83.63%. The SSR markers ORS-453 and CO-306 showed the highest PIC values of 0.76 and 0.74, respectively. The primer ORS-453 amplified allele size of 310 base pairs (bp) for female parent L6 and 320 bp for L11, while for male parents, T1 and T2 had allele size 350 bp and 340 bp, respectively. The hybrids from these parents showed a similar size of alleles with parents, including hybrids L6×T1 (310 bp and 350 bp), L6×T2 (310 bp and 340 bp), and L11×T2 (320 bp and 340 bp). Similarly, the primer CO-306 amplified allele size 350 bp and 330 bp for female parents L6 and L11, respectively, while, allele size 300 bp and 310 bp for male parents T1 and T2, respectively. The hybrids’ allele size was like the parents viz., L6×T1 (350 bp and 300 bp), L6×T2 (350 bp and 310 bp), and L11×T2 (330 bp and 310 bp). All 60 hybrids and their 23 parents were grouped into three main clusters (A, B and C) based upon DARWIN v.6.0 and STRUCTURE v.2.3 Bayesian analyses using genotypic data. Further, each main cluster was divided into two sub-divisions. Each sub-division showed the relatedness of parents and their hybrids, thus authenticating the genetic purity of hybrids. In conclusion, this study provides useful for accurate and effective identification of hybrids, which will help to improve seed genetic purity testing globally.
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spelling pubmed-91194572022-05-20 Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers Ahmed, Hafiz Ghulam Muhu-Din Rizwan, Muhammad Naeem, Muhammad Khan, Muhammad Ahsan Baloch, Faheem Shehzad Sun, Sangmi Chung, Gyuhwa PLoS One Research Article Genetic purity is a prerequisite for exploiting the potential of hybrids in cross-pollinated crops, such as sunflower. In this regard DNA-based study was conducted using 110 simple sequence repeat (SSR) markers to check the genetic purity of 23 parents and their 60 hybrids in sunflower. The polymorphism was shown in 92 markers with value 83.63%. The SSR markers ORS-453 and CO-306 showed the highest PIC values of 0.76 and 0.74, respectively. The primer ORS-453 amplified allele size of 310 base pairs (bp) for female parent L6 and 320 bp for L11, while for male parents, T1 and T2 had allele size 350 bp and 340 bp, respectively. The hybrids from these parents showed a similar size of alleles with parents, including hybrids L6×T1 (310 bp and 350 bp), L6×T2 (310 bp and 340 bp), and L11×T2 (320 bp and 340 bp). Similarly, the primer CO-306 amplified allele size 350 bp and 330 bp for female parents L6 and L11, respectively, while, allele size 300 bp and 310 bp for male parents T1 and T2, respectively. The hybrids’ allele size was like the parents viz., L6×T1 (350 bp and 300 bp), L6×T2 (350 bp and 310 bp), and L11×T2 (330 bp and 310 bp). All 60 hybrids and their 23 parents were grouped into three main clusters (A, B and C) based upon DARWIN v.6.0 and STRUCTURE v.2.3 Bayesian analyses using genotypic data. Further, each main cluster was divided into two sub-divisions. Each sub-division showed the relatedness of parents and their hybrids, thus authenticating the genetic purity of hybrids. In conclusion, this study provides useful for accurate and effective identification of hybrids, which will help to improve seed genetic purity testing globally. Public Library of Science 2022-05-19 /pmc/articles/PMC9119457/ /pubmed/35588423 http://dx.doi.org/10.1371/journal.pone.0267383 Text en © 2022 Ahmed et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ahmed, Hafiz Ghulam Muhu-Din
Rizwan, Muhammad
Naeem, Muhammad
Khan, Muhammad Ahsan
Baloch, Faheem Shehzad
Sun, Sangmi
Chung, Gyuhwa
Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers
title Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers
title_full Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers
title_fullStr Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers
title_full_unstemmed Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers
title_short Molecular characterization and validation of sunflower (Helianthus annuus L.) hybrids through SSR markers
title_sort molecular characterization and validation of sunflower (helianthus annuus l.) hybrids through ssr markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119457/
https://www.ncbi.nlm.nih.gov/pubmed/35588423
http://dx.doi.org/10.1371/journal.pone.0267383
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