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Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
Appropriate cardiac performance depends on a tightly controlled handling of Ca(2+) in a broad range of species, from invertebrates to mammals. The role of the Ca(2+) ATPase, SERCA, in Ca(2+) handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119464/ https://www.ncbi.nlm.nih.gov/pubmed/35588119 http://dx.doi.org/10.1371/journal.pone.0267156 |
Sumario: | Appropriate cardiac performance depends on a tightly controlled handling of Ca(2+) in a broad range of species, from invertebrates to mammals. The role of the Ca(2+) ATPase, SERCA, in Ca(2+) handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein, we give evidence that 4E binding protein (4E-BP) is a novel regulator of SERCA activity in Drosophila melanogaster during cardiac function. Flies over-expressing 4E-BP showed improved cardiac performance in young individuals associated with incremented SERCA activity. Moreover, we demonstrate that SERCA interacts with translation initiation factors eIF4E-1, eIF4E-2 and eIF4E-4 in a yeast two-hybrid assay. The specific identification of eIF4E-4 in cardiac tissue leads us to propose that the interaction of elF4E-4 with SERCA may be the basis of the cardiac effects observed in 4E-BP over-expressing flies associated with incremented SERCA activity. |
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