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Molecular spikes: a gold standard for single-cell RNA counting

Single-cell sequencing methods rely on molecule-counting strategies to account for amplification biases, yet no experimental strategy to evaluate counting performance exists. Here, we introduce molecular spikes—RNA spike-ins containing built-in unique molecular identifiers (UMIs) that we use to iden...

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Detalles Bibliográficos
Autores principales:	Ziegenhain, Christoph, Hendriks, Gert-Jan, Hagemann-Jensen, Michael, Sandberg, Rickard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group US 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119855/ https://www.ncbi.nlm.nih.gov/pubmed/35468967 http://dx.doi.org/10.1038/s41592-022-01446-x

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