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Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach

The assessment of tissue chromophores at a volumetric scale is vital for an improved diagnosis and treatment of a large number of diseases. Spectral photoacoustic imaging (sPAI) co-registered with high-resolution ultrasound (US) is an innovative technology that has a great potential for clinical tra...

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Autores principales: Grasso, Valeria, Willumeit-Rӧmer, Regine, Jose, Jithin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120071/
https://www.ncbi.nlm.nih.gov/pubmed/35601933
http://dx.doi.org/10.1016/j.pacs.2022.100367
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author Grasso, Valeria
Willumeit-Rӧmer, Regine
Jose, Jithin
author_facet Grasso, Valeria
Willumeit-Rӧmer, Regine
Jose, Jithin
author_sort Grasso, Valeria
collection PubMed
description The assessment of tissue chromophores at a volumetric scale is vital for an improved diagnosis and treatment of a large number of diseases. Spectral photoacoustic imaging (sPAI) co-registered with high-resolution ultrasound (US) is an innovative technology that has a great potential for clinical translation as it can assess the volumetric distribution of the tissue components. Conventionally, to detect and separate the chromophores from sPAI, an input of the expected tissue absorption spectra is required. However, in pathological conditions, the prediction of the absorption spectra is difficult as it can change with respect to the physiological state. Besides, this conventional approach can also be hampered due to spectral coloring, which is a prominent distortion effect that induces spectral changes at depth. Here, we are proposing a novel data-driven framework that can overcome all these limitations and provide an improved assessment of the tissue chromophores. We have developed a superpixel spectral unmixing (SPAX) approach that can detect the most and less prominent absorber spectra and their volumetric distribution without any user interactions. Within the SPAX framework, we have also implemented an advanced spectral coloring compensation approach by utilizing US image segmentation and Monte Carlo simulations, based on a predefined library of optical properties. The framework has been tested on tissue-mimicking phantoms and also on healthy animals. The obtained results show enhanced specificity and sensitivity for the detection of tissue chromophores. To our knowledge, this is a unique framework that accounts for the spectral coloring and provides automated detection of tissue spectral signatures at a volumetric scale, which can open many possibilities for translational research.
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spelling pubmed-91200712022-05-21 Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach Grasso, Valeria Willumeit-Rӧmer, Regine Jose, Jithin Photoacoustics Research Article The assessment of tissue chromophores at a volumetric scale is vital for an improved diagnosis and treatment of a large number of diseases. Spectral photoacoustic imaging (sPAI) co-registered with high-resolution ultrasound (US) is an innovative technology that has a great potential for clinical translation as it can assess the volumetric distribution of the tissue components. Conventionally, to detect and separate the chromophores from sPAI, an input of the expected tissue absorption spectra is required. However, in pathological conditions, the prediction of the absorption spectra is difficult as it can change with respect to the physiological state. Besides, this conventional approach can also be hampered due to spectral coloring, which is a prominent distortion effect that induces spectral changes at depth. Here, we are proposing a novel data-driven framework that can overcome all these limitations and provide an improved assessment of the tissue chromophores. We have developed a superpixel spectral unmixing (SPAX) approach that can detect the most and less prominent absorber spectra and their volumetric distribution without any user interactions. Within the SPAX framework, we have also implemented an advanced spectral coloring compensation approach by utilizing US image segmentation and Monte Carlo simulations, based on a predefined library of optical properties. The framework has been tested on tissue-mimicking phantoms and also on healthy animals. The obtained results show enhanced specificity and sensitivity for the detection of tissue chromophores. To our knowledge, this is a unique framework that accounts for the spectral coloring and provides automated detection of tissue spectral signatures at a volumetric scale, which can open many possibilities for translational research. Elsevier 2022-05-11 /pmc/articles/PMC9120071/ /pubmed/35601933 http://dx.doi.org/10.1016/j.pacs.2022.100367 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Grasso, Valeria
Willumeit-Rӧmer, Regine
Jose, Jithin
Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach
title Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach
title_full Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach
title_fullStr Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach
title_full_unstemmed Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach
title_short Superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: A photoacoustic data-driven approach
title_sort superpixel spectral unmixing framework for the volumetric assessment of tissue chromophores: a photoacoustic data-driven approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120071/
https://www.ncbi.nlm.nih.gov/pubmed/35601933
http://dx.doi.org/10.1016/j.pacs.2022.100367
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