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A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence
Enterohemorrhagic Escherichia coli (EHEC) is one of the most common foodborne pathogens. However, no drug that prevents the severe complications caused by this bacterium has been approved yet. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC(150)) adsorbs Sh...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120352/ https://www.ncbi.nlm.nih.gov/pubmed/35602086 http://dx.doi.org/10.3389/fmicb.2022.883689 |
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author | Hirakawa, Hidetada Suzue, Kazutomo Uchida, Motoyuki Takita, Ayako Kamitani, Wataru Tomita, Haruyoshi |
author_facet | Hirakawa, Hidetada Suzue, Kazutomo Uchida, Motoyuki Takita, Ayako Kamitani, Wataru Tomita, Haruyoshi |
author_sort | Hirakawa, Hidetada |
collection | PubMed |
description | Enterohemorrhagic Escherichia coli (EHEC) is one of the most common foodborne pathogens. However, no drug that prevents the severe complications caused by this bacterium has been approved yet. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC(150)) adsorbs Shiga toxins, and Type III secretory EspA/EspB proteins responsible for EHEC pathogenesis, and decreases the extracellular levels of these proteins. On the other hand, this material did not affect the growth of EHEC. Citrobacter rodentium traditionally used to estimate Type III secretion system-associated virulence in mice is highly virulent. The survival period of infected mice was prolonged when MgOC(150) was administered. This adsorbent disturbed neither mammalian cells nor normal intestinal bacteria, such as Enterococcus hirae, Lactobacillus acidophilus, and Lactobacillus casei. In contrast, MgOC(150) adsorbed antimicrobial agents, including β-lactams, quinolones, tetracyclines, and trimethoprim/sulfamethoxazole. However, fosfomycin and amikacin were not adsorbed. Thus, MgOC(150) can be used with fosfomycin and amikacin to treat infections. MgOC(150) is used for industrial purposes, such as an electrode catalyst, a bioelectrode, and enzyme immobilization. The study proposed another potential application of MgOC(150), assisting anti-EHEC chemotherapy. |
format | Online Article Text |
id | pubmed-9120352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91203522022-05-21 A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence Hirakawa, Hidetada Suzue, Kazutomo Uchida, Motoyuki Takita, Ayako Kamitani, Wataru Tomita, Haruyoshi Front Microbiol Microbiology Enterohemorrhagic Escherichia coli (EHEC) is one of the most common foodborne pathogens. However, no drug that prevents the severe complications caused by this bacterium has been approved yet. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC(150)) adsorbs Shiga toxins, and Type III secretory EspA/EspB proteins responsible for EHEC pathogenesis, and decreases the extracellular levels of these proteins. On the other hand, this material did not affect the growth of EHEC. Citrobacter rodentium traditionally used to estimate Type III secretion system-associated virulence in mice is highly virulent. The survival period of infected mice was prolonged when MgOC(150) was administered. This adsorbent disturbed neither mammalian cells nor normal intestinal bacteria, such as Enterococcus hirae, Lactobacillus acidophilus, and Lactobacillus casei. In contrast, MgOC(150) adsorbed antimicrobial agents, including β-lactams, quinolones, tetracyclines, and trimethoprim/sulfamethoxazole. However, fosfomycin and amikacin were not adsorbed. Thus, MgOC(150) can be used with fosfomycin and amikacin to treat infections. MgOC(150) is used for industrial purposes, such as an electrode catalyst, a bioelectrode, and enzyme immobilization. The study proposed another potential application of MgOC(150), assisting anti-EHEC chemotherapy. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9120352/ /pubmed/35602086 http://dx.doi.org/10.3389/fmicb.2022.883689 Text en Copyright © 2022 Hirakawa, Suzue, Uchida, Takita, Kamitani and Tomita. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Hirakawa, Hidetada Suzue, Kazutomo Uchida, Motoyuki Takita, Ayako Kamitani, Wataru Tomita, Haruyoshi A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence |
title | A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence |
title_full | A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence |
title_fullStr | A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence |
title_full_unstemmed | A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence |
title_short | A Macroporous Magnesium Oxide-Templated Carbon Adsorbs Shiga Toxins and Type III Secretory Proteins in Enterohemorrhagic Escherichia coli, Which Attenuates Virulence |
title_sort | macroporous magnesium oxide-templated carbon adsorbs shiga toxins and type iii secretory proteins in enterohemorrhagic escherichia coli, which attenuates virulence |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120352/ https://www.ncbi.nlm.nih.gov/pubmed/35602086 http://dx.doi.org/10.3389/fmicb.2022.883689 |
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