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Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory

BACKGROUND: Culture of Mycobacterium tuberculosis remains the gold standard in mycobacteriology laboratories, constrained by the very high risk of contamination; therefore, contamination rate is an important key performance indicator (KPI) for laboratory monitoring and evaluation processes. AIM: Thi...

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Autores principales: Mohammed Adam, Muatsim Ahmed, Ebraheem, Rasha Sayed Mohammed, Bedri, Shahinaz Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120365/
https://www.ncbi.nlm.nih.gov/pubmed/35602070
http://dx.doi.org/10.3389/fmicb.2022.789725
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author Mohammed Adam, Muatsim Ahmed
Ebraheem, Rasha Sayed Mohammed
Bedri, Shahinaz Ahmed
author_facet Mohammed Adam, Muatsim Ahmed
Ebraheem, Rasha Sayed Mohammed
Bedri, Shahinaz Ahmed
author_sort Mohammed Adam, Muatsim Ahmed
collection PubMed
description BACKGROUND: Culture of Mycobacterium tuberculosis remains the gold standard in mycobacteriology laboratories, constrained by the very high risk of contamination; therefore, contamination rate is an important key performance indicator (KPI) for laboratory monitoring and evaluation processes. AIM: This study aimed to investigate the factors that contribute to elevated contamination rates in the Sudan National Tuberculosis Reference Laboratory. METHOD: A laboratory-based retrospective study was applied; a TB culture register-book was carefully reviewed and data from 2 January 2019 to 31 December 2019 were entered, cleaned, and analyzed using IBM SPSS 20. A multivariate logistic regression model was performed to examine two dependent variables, the massive contamination, and the single tube contamination against predictors of reason for cultivation, type of specimen, experiment team, and the quarter of cultivation. RESULTS: It has been found that in 2019 contamination rates were frequently higher; the highest rates were recorded in January and November, 28.2 and 25.2%, respectively. August is an exception with an accepted contamination rate of 4.6%. Of 1,149 specimens requested for culture, 945 (82.2%) samples were eligible to be included in multivariate logistic regression analysis. The team conducting the experiment was significantly associated with a high single tube contamination p value 0.007; adjusted odds ratio AOR 3.570 (1.415–9.005). The correlation between the single tube contamination and the massive contamination is significant; p value 0.01. CONCLUSION: The study concludes that high culture contamination is the greatest risk to the quality of laboratory service and can end in either the loss of specimens or delay in the decisions of initiating patient treatment. In addition, the low quality or incompleteness of data increases the uncertainty and undermines the measurement of key performance indicators.
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spelling pubmed-91203652022-05-21 Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory Mohammed Adam, Muatsim Ahmed Ebraheem, Rasha Sayed Mohammed Bedri, Shahinaz Ahmed Front Microbiol Microbiology BACKGROUND: Culture of Mycobacterium tuberculosis remains the gold standard in mycobacteriology laboratories, constrained by the very high risk of contamination; therefore, contamination rate is an important key performance indicator (KPI) for laboratory monitoring and evaluation processes. AIM: This study aimed to investigate the factors that contribute to elevated contamination rates in the Sudan National Tuberculosis Reference Laboratory. METHOD: A laboratory-based retrospective study was applied; a TB culture register-book was carefully reviewed and data from 2 January 2019 to 31 December 2019 were entered, cleaned, and analyzed using IBM SPSS 20. A multivariate logistic regression model was performed to examine two dependent variables, the massive contamination, and the single tube contamination against predictors of reason for cultivation, type of specimen, experiment team, and the quarter of cultivation. RESULTS: It has been found that in 2019 contamination rates were frequently higher; the highest rates were recorded in January and November, 28.2 and 25.2%, respectively. August is an exception with an accepted contamination rate of 4.6%. Of 1,149 specimens requested for culture, 945 (82.2%) samples were eligible to be included in multivariate logistic regression analysis. The team conducting the experiment was significantly associated with a high single tube contamination p value 0.007; adjusted odds ratio AOR 3.570 (1.415–9.005). The correlation between the single tube contamination and the massive contamination is significant; p value 0.01. CONCLUSION: The study concludes that high culture contamination is the greatest risk to the quality of laboratory service and can end in either the loss of specimens or delay in the decisions of initiating patient treatment. In addition, the low quality or incompleteness of data increases the uncertainty and undermines the measurement of key performance indicators. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9120365/ /pubmed/35602070 http://dx.doi.org/10.3389/fmicb.2022.789725 Text en Copyright © 2022 Mohammed Adam, Ebraheem and Bedri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mohammed Adam, Muatsim Ahmed
Ebraheem, Rasha Sayed Mohammed
Bedri, Shahinaz Ahmed
Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory
title Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory
title_full Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory
title_fullStr Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory
title_full_unstemmed Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory
title_short Statistical Investigation of High Culture Contamination Rates in Mycobacteriology Laboratory
title_sort statistical investigation of high culture contamination rates in mycobacteriology laboratory
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120365/
https://www.ncbi.nlm.nih.gov/pubmed/35602070
http://dx.doi.org/10.3389/fmicb.2022.789725
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