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Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach

To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as p...

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Autores principales: Solmi, Leandro, Rosli, Hernán G., Pombo, Marina A., Stalder, Santiago, Rossi, Franco R., Romero, Fernando M., Ruiz, Oscar A., Gárriz, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120772/
https://www.ncbi.nlm.nih.gov/pubmed/35602047
http://dx.doi.org/10.3389/fmicb.2022.893626
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author Solmi, Leandro
Rosli, Hernán G.
Pombo, Marina A.
Stalder, Santiago
Rossi, Franco R.
Romero, Fernando M.
Ruiz, Oscar A.
Gárriz, Andrés
author_facet Solmi, Leandro
Rosli, Hernán G.
Pombo, Marina A.
Stalder, Santiago
Rossi, Franco R.
Romero, Fernando M.
Ruiz, Oscar A.
Gárriz, Andrés
author_sort Solmi, Leandro
collection PubMed
description To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as polyamines have attracted considerable attention as they are involved in important cellular processes, but it is not known yet how phytopathogenic bacteria regulate polyamine homeostasis in the plant environment. In the present study, we performed a meta-analysis of publicly available transcriptomic data from experiments conducted on bacteria to begin delving into this topic and better understand the regulation of polyamine metabolism and its links to pathogenicity. We focused our research on Pseudomonas syringae, an important phytopathogen that causes disease in many economically valuable plant species. Our analysis discovered that polyamine synthesis, as well as general gene expression activation and energy production are induced in the early stages of the disease. On the contrary, synthesis of these compounds is inhibited whereas its transport is upregulated later in the process, which correlates with the induction of virulence genes and the metabolism of nitrogen and carboxylic acids. We also found that activation of plant defense mechanisms affects bacterial polyamine synthesis to some extent, which could reduce bacterial cell fitness in the plant environment. Furthermore, data suggest that a proper bacterial response to oxidative conditions requires a decrease in polyamine production. The implications of these findings are discussed.
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spelling pubmed-91207722022-05-21 Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach Solmi, Leandro Rosli, Hernán G. Pombo, Marina A. Stalder, Santiago Rossi, Franco R. Romero, Fernando M. Ruiz, Oscar A. Gárriz, Andrés Front Microbiol Microbiology To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as polyamines have attracted considerable attention as they are involved in important cellular processes, but it is not known yet how phytopathogenic bacteria regulate polyamine homeostasis in the plant environment. In the present study, we performed a meta-analysis of publicly available transcriptomic data from experiments conducted on bacteria to begin delving into this topic and better understand the regulation of polyamine metabolism and its links to pathogenicity. We focused our research on Pseudomonas syringae, an important phytopathogen that causes disease in many economically valuable plant species. Our analysis discovered that polyamine synthesis, as well as general gene expression activation and energy production are induced in the early stages of the disease. On the contrary, synthesis of these compounds is inhibited whereas its transport is upregulated later in the process, which correlates with the induction of virulence genes and the metabolism of nitrogen and carboxylic acids. We also found that activation of plant defense mechanisms affects bacterial polyamine synthesis to some extent, which could reduce bacterial cell fitness in the plant environment. Furthermore, data suggest that a proper bacterial response to oxidative conditions requires a decrease in polyamine production. The implications of these findings are discussed. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9120772/ /pubmed/35602047 http://dx.doi.org/10.3389/fmicb.2022.893626 Text en Copyright © 2022 Solmi, Rosli, Pombo, Stalder, Rossi, Romero, Ruiz and Gárriz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Solmi, Leandro
Rosli, Hernán G.
Pombo, Marina A.
Stalder, Santiago
Rossi, Franco R.
Romero, Fernando M.
Ruiz, Oscar A.
Gárriz, Andrés
Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_full Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_fullStr Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_full_unstemmed Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_short Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
title_sort inferring the significance of the polyamine metabolism in the phytopathogenic bacteria pseudomonas syringae: a meta-analysis approach
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120772/
https://www.ncbi.nlm.nih.gov/pubmed/35602047
http://dx.doi.org/10.3389/fmicb.2022.893626
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