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SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40–80%. SdiA acts as a quorum sensing regulator in many foodborne pathogens, but its role in C. sakazakii remains unclear. Here, we further determined the effect of the sdiA gene in C. sakazak...

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Autores principales: Cheng, Chuansong, Yan, Xiaotong, Liu, Binxiong, Jiang, Tao, Zhou, Ziwen, Guo, Fengting, Zhang, Qianwen, Li, Changcheng, Fang, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120920/
https://www.ncbi.nlm.nih.gov/pubmed/35602061
http://dx.doi.org/10.3389/fmicb.2022.901912
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author Cheng, Chuansong
Yan, Xiaotong
Liu, Binxiong
Jiang, Tao
Zhou, Ziwen
Guo, Fengting
Zhang, Qianwen
Li, Changcheng
Fang, Ting
author_facet Cheng, Chuansong
Yan, Xiaotong
Liu, Binxiong
Jiang, Tao
Zhou, Ziwen
Guo, Fengting
Zhang, Qianwen
Li, Changcheng
Fang, Ting
author_sort Cheng, Chuansong
collection PubMed
description Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40–80%. SdiA acts as a quorum sensing regulator in many foodborne pathogens, but its role in C. sakazakii remains unclear. Here, we further determined the effect of the sdiA gene in C. sakazakii pathogenicity. The SdiA gene in C. sakazakii was knocked out by gene editing technology, and the biological characteristics of the ΔsdiA mutant of C. sakazakii were studied, followed by transcriptome analysis to elucidate its effects. The results suggested that SdiA gene enhanced the drug resistance of C. sakazakii but diminished its motility, adhesion and biofilm formation ability and had no effect on its growth. Transcriptome analysis showed that the ΔsdiA upregulated the expression levels of D-galactose operon genes (including dgoR, dgoK, dgoA, dgoD and dgoT) and flagella-related genes (FliA and FliC) in C. sakazakii and downregulated the expression levels of related genes in the type VI secretion system (VasK gene was downregulated by 1.53-fold) and ABC transport system (downregulated by 1.5-fold), indicating that SdiA gene was related to the physiological metabolism of C. sakazakii. The results were useful for clarifying the pathogenic mechanism of C. sakazakii and provide a theoretical basis for controlling bacterial infection.
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spelling pubmed-91209202022-05-21 SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation Cheng, Chuansong Yan, Xiaotong Liu, Binxiong Jiang, Tao Zhou, Ziwen Guo, Fengting Zhang, Qianwen Li, Changcheng Fang, Ting Front Microbiol Microbiology Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40–80%. SdiA acts as a quorum sensing regulator in many foodborne pathogens, but its role in C. sakazakii remains unclear. Here, we further determined the effect of the sdiA gene in C. sakazakii pathogenicity. The SdiA gene in C. sakazakii was knocked out by gene editing technology, and the biological characteristics of the ΔsdiA mutant of C. sakazakii were studied, followed by transcriptome analysis to elucidate its effects. The results suggested that SdiA gene enhanced the drug resistance of C. sakazakii but diminished its motility, adhesion and biofilm formation ability and had no effect on its growth. Transcriptome analysis showed that the ΔsdiA upregulated the expression levels of D-galactose operon genes (including dgoR, dgoK, dgoA, dgoD and dgoT) and flagella-related genes (FliA and FliC) in C. sakazakii and downregulated the expression levels of related genes in the type VI secretion system (VasK gene was downregulated by 1.53-fold) and ABC transport system (downregulated by 1.5-fold), indicating that SdiA gene was related to the physiological metabolism of C. sakazakii. The results were useful for clarifying the pathogenic mechanism of C. sakazakii and provide a theoretical basis for controlling bacterial infection. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9120920/ /pubmed/35602061 http://dx.doi.org/10.3389/fmicb.2022.901912 Text en Copyright © 2022 Cheng, Yan, Liu, Jiang, Zhou, Guo, Zhang, Li and Fang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Cheng, Chuansong
Yan, Xiaotong
Liu, Binxiong
Jiang, Tao
Zhou, Ziwen
Guo, Fengting
Zhang, Qianwen
Li, Changcheng
Fang, Ting
SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
title SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
title_full SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
title_fullStr SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
title_full_unstemmed SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
title_short SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation
title_sort sdia enhanced the drug resistance of cronobacter sakazakii and suppressed its motility, adhesion and biofilm formation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120920/
https://www.ncbi.nlm.nih.gov/pubmed/35602061
http://dx.doi.org/10.3389/fmicb.2022.901912
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