Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates

Heat stress triggers a specific set of proteins in budding yeast to form solid-like biomolecular condensates, which are dispersed by molecular chaperones. Here, we describe a protocol to study the kinetics of chaperone-facilitated condensate dispersal using biochemical reconstitution and fluorescenc...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoo, Haneul, Drummond, D. Allan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121323/
https://www.ncbi.nlm.nih.gov/pubmed/35600925
http://dx.doi.org/10.1016/j.xpro.2022.101409

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121323/
https://www.ncbi.nlm.nih.gov/pubmed/35600925
http://dx.doi.org/10.1016/j.xpro.2022.101409

Ejemplares similares