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Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates
Heat stress triggers a specific set of proteins in budding yeast to form solid-like biomolecular condensates, which are dispersed by molecular chaperones. Here, we describe a protocol to study the kinetics of chaperone-facilitated condensate dispersal using biochemical reconstitution and fluorescenc...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121323/ https://www.ncbi.nlm.nih.gov/pubmed/35600925 http://dx.doi.org/10.1016/j.xpro.2022.101409 |