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Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins

Commensal bacteria are known to promote host growth. Such effect partly relies on the capacity of microbes to regulate the host’s transcriptional response. However, these evidences mainly come from comparing the transcriptional response caused by commensal bacteria with that of axenic animals, makin...

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Autores principales: Gallo, Marialaura, Vento, Justin M., Joncour, Pauline, Quagliariello, Andrea, Maritan, Elisa, Silva-Soares, Nuno F., Battistolli, Matteo, Beisel, Chase L., Martino, Maria Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121327/
https://www.ncbi.nlm.nih.gov/pubmed/35601912
http://dx.doi.org/10.1016/j.isci.2022.104357
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author Gallo, Marialaura
Vento, Justin M.
Joncour, Pauline
Quagliariello, Andrea
Maritan, Elisa
Silva-Soares, Nuno F.
Battistolli, Matteo
Beisel, Chase L.
Martino, Maria Elena
author_facet Gallo, Marialaura
Vento, Justin M.
Joncour, Pauline
Quagliariello, Andrea
Maritan, Elisa
Silva-Soares, Nuno F.
Battistolli, Matteo
Beisel, Chase L.
Martino, Maria Elena
author_sort Gallo, Marialaura
collection PubMed
description Commensal bacteria are known to promote host growth. Such effect partly relies on the capacity of microbes to regulate the host’s transcriptional response. However, these evidences mainly come from comparing the transcriptional response caused by commensal bacteria with that of axenic animals, making it difficult to identify the animal genes that are specifically regulated by beneficial microbes. Here, we employ Drosophila melanogaster associated with Lactiplantibacillus plantarum to understand the host genetic pathways regulated by beneficial bacteria and leading to improved host growth. We show that microbial benefit to the host relies on the downregulation of peptidoglycan-recognition proteins. Specifically, we report that bacterial proliferation triggers the lower expression of PGRP-SC1 in larval midgut, which ultimately leads to improved host growth and development. Our study helps elucidate the mechanisms underlying the beneficial effect exerted by commensal bacteria, defining the role of immune effectors in the relationship between Drosophila and its gut microbes.
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spelling pubmed-91213272022-05-21 Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins Gallo, Marialaura Vento, Justin M. Joncour, Pauline Quagliariello, Andrea Maritan, Elisa Silva-Soares, Nuno F. Battistolli, Matteo Beisel, Chase L. Martino, Maria Elena iScience Article Commensal bacteria are known to promote host growth. Such effect partly relies on the capacity of microbes to regulate the host’s transcriptional response. However, these evidences mainly come from comparing the transcriptional response caused by commensal bacteria with that of axenic animals, making it difficult to identify the animal genes that are specifically regulated by beneficial microbes. Here, we employ Drosophila melanogaster associated with Lactiplantibacillus plantarum to understand the host genetic pathways regulated by beneficial bacteria and leading to improved host growth. We show that microbial benefit to the host relies on the downregulation of peptidoglycan-recognition proteins. Specifically, we report that bacterial proliferation triggers the lower expression of PGRP-SC1 in larval midgut, which ultimately leads to improved host growth and development. Our study helps elucidate the mechanisms underlying the beneficial effect exerted by commensal bacteria, defining the role of immune effectors in the relationship between Drosophila and its gut microbes. Elsevier 2022-05-05 /pmc/articles/PMC9121327/ /pubmed/35601912 http://dx.doi.org/10.1016/j.isci.2022.104357 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Gallo, Marialaura
Vento, Justin M.
Joncour, Pauline
Quagliariello, Andrea
Maritan, Elisa
Silva-Soares, Nuno F.
Battistolli, Matteo
Beisel, Chase L.
Martino, Maria Elena
Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins
title Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins
title_full Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins
title_fullStr Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins
title_full_unstemmed Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins
title_short Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins
title_sort beneficial commensal bacteria promote drosophila growth by downregulating the expression of peptidoglycan recognition proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121327/
https://www.ncbi.nlm.nih.gov/pubmed/35601912
http://dx.doi.org/10.1016/j.isci.2022.104357
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