Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-Methadone for Use in Cells, Organelles, and Biofluids

[Image: see text] We report a reagentless, intensity-based S-methadone fluorescent sensor, iS-methadoneSnFR, consisting of a circularly permuted GFP inserted within the sequence of a mutated bacterial periplasmic binding protein (PBP). We evolved a previously reported nicotine-binding PBP to become...

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Detalles Bibliográficos
Autores principales: Muthusamy, Anand K., Kim, Charlene H., Virgil, Scott C., Knox, Hailey J., Marvin, Jonathan S., Nichols, Aaron L., Cohen, Bruce N., Dougherty, Dennis A., Looger, Loren L., Lester, Henry A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121368/
https://www.ncbi.nlm.nih.gov/pubmed/35446570
http://dx.doi.org/10.1021/jacs.2c02323

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