Cargando…
Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing
BACKGROUND: The aim of our study was to use the differentially expressed mRNAs (DEmRNAs) and differentially expressed miRNAs (DEmiRNAs) to illustrate the underlying mechanism of hypoxia in liver cancer. METHODS: In this study, a cell model of hypoxia was established, and autophagy activity was measu...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121577/ https://www.ncbi.nlm.nih.gov/pubmed/35590240 http://dx.doi.org/10.1186/s12863-022-01055-9 |
_version_ | 1784711181738442752 |
---|---|
author | Zhao, Xin Liu, Wenpeng Liu, Baowang Zeng, Qiang Cui, Ziqiang Wang, Yang Cao, Jinglin Gao, Qingjun Zhao, Caiyan Dou, Jian |
author_facet | Zhao, Xin Liu, Wenpeng Liu, Baowang Zeng, Qiang Cui, Ziqiang Wang, Yang Cao, Jinglin Gao, Qingjun Zhao, Caiyan Dou, Jian |
author_sort | Zhao, Xin |
collection | PubMed |
description | BACKGROUND: The aim of our study was to use the differentially expressed mRNAs (DEmRNAs) and differentially expressed miRNAs (DEmiRNAs) to illustrate the underlying mechanism of hypoxia in liver cancer. METHODS: In this study, a cell model of hypoxia was established, and autophagy activity was measured with western blotting and transmission electron microscopy. The effect of hypoxia conditions on the invasion of liver cancer cell was evaluated. RNA sequencing was used to identify DEmRNAs and DEmiRNAs to explore the mechanism of hypoxia in liver cancer cells. RESULTS: We found that autophagy activation was triggered by hypoxia stress and hypoxia might promote liver cancer cell invasion. In addition, a total of 407 shared DEmRNAs and 57 shared DEmiRNAs were identified in both HCCLM3 hypoxia group and SMMC-7721 hypoxia group compared with control group. Furthermore, 278 DEmRNAs and 24 DEmiRNAs were identified as cancer hypoxia-specific DEmRNAs and DEmiRNAs. Finally, we obtained 19 DEmiRNAs with high degree based on the DEmiRNA-DEmRNA interaction network. Among them, hsa-miR-483-5p, hsa-miR-4739, hsa-miR-214-3p and hsa-miR-296-5p may be potential gene signatures related to liver cancer hypoxia. CONCLUSIONS: Our study may help to understand the potential molecular mechanism of hypoxia in liver cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12863-022-01055-9. |
format | Online Article Text |
id | pubmed-9121577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-91215772022-05-21 Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing Zhao, Xin Liu, Wenpeng Liu, Baowang Zeng, Qiang Cui, Ziqiang Wang, Yang Cao, Jinglin Gao, Qingjun Zhao, Caiyan Dou, Jian BMC Genom Data Research BACKGROUND: The aim of our study was to use the differentially expressed mRNAs (DEmRNAs) and differentially expressed miRNAs (DEmiRNAs) to illustrate the underlying mechanism of hypoxia in liver cancer. METHODS: In this study, a cell model of hypoxia was established, and autophagy activity was measured with western blotting and transmission electron microscopy. The effect of hypoxia conditions on the invasion of liver cancer cell was evaluated. RNA sequencing was used to identify DEmRNAs and DEmiRNAs to explore the mechanism of hypoxia in liver cancer cells. RESULTS: We found that autophagy activation was triggered by hypoxia stress and hypoxia might promote liver cancer cell invasion. In addition, a total of 407 shared DEmRNAs and 57 shared DEmiRNAs were identified in both HCCLM3 hypoxia group and SMMC-7721 hypoxia group compared with control group. Furthermore, 278 DEmRNAs and 24 DEmiRNAs were identified as cancer hypoxia-specific DEmRNAs and DEmiRNAs. Finally, we obtained 19 DEmiRNAs with high degree based on the DEmiRNA-DEmRNA interaction network. Among them, hsa-miR-483-5p, hsa-miR-4739, hsa-miR-214-3p and hsa-miR-296-5p may be potential gene signatures related to liver cancer hypoxia. CONCLUSIONS: Our study may help to understand the potential molecular mechanism of hypoxia in liver cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12863-022-01055-9. BioMed Central 2022-05-19 /pmc/articles/PMC9121577/ /pubmed/35590240 http://dx.doi.org/10.1186/s12863-022-01055-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhao, Xin Liu, Wenpeng Liu, Baowang Zeng, Qiang Cui, Ziqiang Wang, Yang Cao, Jinglin Gao, Qingjun Zhao, Caiyan Dou, Jian Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing |
title | Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing |
title_full | Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing |
title_fullStr | Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing |
title_full_unstemmed | Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing |
title_short | Exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on RNA sequencing |
title_sort | exploring the underlying molecular mechanism of liver cancer cells under hypoxia based on rna sequencing |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121577/ https://www.ncbi.nlm.nih.gov/pubmed/35590240 http://dx.doi.org/10.1186/s12863-022-01055-9 |
work_keys_str_mv | AT zhaoxin exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT liuwenpeng exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT liubaowang exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT zengqiang exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT cuiziqiang exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT wangyang exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT caojinglin exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT gaoqingjun exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT zhaocaiyan exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing AT doujian exploringtheunderlyingmolecularmechanismoflivercancercellsunderhypoxiabasedonrnasequencing |