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Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor

Surface-enhanced Raman scattering (SERS)-based assays have been recently developed to overcome the low detection sensitivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SERS-based assays using magnetic beads in microtubes slightly improved the limit of detection (LoD) for SARS-C...

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Autores principales: Park, Sohyun, Su Jeon, Chang, Choi, Namhyun, Moon, Joung-Il, Min Lee, Kang, Hyun Pyun, Sung, Kang, Taejoon, Choo, Jaebum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121656/
https://www.ncbi.nlm.nih.gov/pubmed/35611066
http://dx.doi.org/10.1016/j.cej.2022.137085
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author Park, Sohyun
Su Jeon, Chang
Choi, Namhyun
Moon, Joung-Il
Min Lee, Kang
Hyun Pyun, Sung
Kang, Taejoon
Choo, Jaebum
author_facet Park, Sohyun
Su Jeon, Chang
Choi, Namhyun
Moon, Joung-Il
Min Lee, Kang
Hyun Pyun, Sung
Kang, Taejoon
Choo, Jaebum
author_sort Park, Sohyun
collection PubMed
description Surface-enhanced Raman scattering (SERS)-based assays have been recently developed to overcome the low detection sensitivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SERS-based assays using magnetic beads in microtubes slightly improved the limit of detection (LoD) for SARS-CoV-2. However, the sensitivity and reproducibility of the method are still insufficient for reliable SARS-CoV-2 detection. In this study, we developed a SERS-based microdroplet sensor to dramatically improve the LoD and reproducibility of SARS-CoV-2 detection. Raman signals were measured for SERS nanotags in 140 droplets passing through a laser focal volume fixed at the center of the channel for 15 s. A comparison of the Raman signals of SERS nanotags measured in a microtube with those measured for multiple droplets in the microfluidic channel revealed that the LoD and coefficient of variation significantly improved from 36 to 0.22 PFU/mL and 21.2% to 1.79%, respectively. This improvement resulted from the ensemble average effects because the signals were measured for SERS nanotags in multiple droplets. Moreover, the total assay time decreased from 30 to 10 min. A clinical test was performed on patient samples to evaluate the clinical efficacy of the SERS-based microdroplet sensor. The assay results agreed well with those measured by the reverse transcription-polymerase chain reaction (RT-PCR) method. The proposed SERS-based microdroplet sensor is expected to be used as a new point-of-care diagnostic platform for quick and accurate detection of SARS-CoV-2 in the field.
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spelling pubmed-91216562022-05-20 Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor Park, Sohyun Su Jeon, Chang Choi, Namhyun Moon, Joung-Il Min Lee, Kang Hyun Pyun, Sung Kang, Taejoon Choo, Jaebum Chem Eng J Article Surface-enhanced Raman scattering (SERS)-based assays have been recently developed to overcome the low detection sensitivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SERS-based assays using magnetic beads in microtubes slightly improved the limit of detection (LoD) for SARS-CoV-2. However, the sensitivity and reproducibility of the method are still insufficient for reliable SARS-CoV-2 detection. In this study, we developed a SERS-based microdroplet sensor to dramatically improve the LoD and reproducibility of SARS-CoV-2 detection. Raman signals were measured for SERS nanotags in 140 droplets passing through a laser focal volume fixed at the center of the channel for 15 s. A comparison of the Raman signals of SERS nanotags measured in a microtube with those measured for multiple droplets in the microfluidic channel revealed that the LoD and coefficient of variation significantly improved from 36 to 0.22 PFU/mL and 21.2% to 1.79%, respectively. This improvement resulted from the ensemble average effects because the signals were measured for SERS nanotags in multiple droplets. Moreover, the total assay time decreased from 30 to 10 min. A clinical test was performed on patient samples to evaluate the clinical efficacy of the SERS-based microdroplet sensor. The assay results agreed well with those measured by the reverse transcription-polymerase chain reaction (RT-PCR) method. The proposed SERS-based microdroplet sensor is expected to be used as a new point-of-care diagnostic platform for quick and accurate detection of SARS-CoV-2 in the field. Elsevier B.V. 2022-10-15 2022-05-20 /pmc/articles/PMC9121656/ /pubmed/35611066 http://dx.doi.org/10.1016/j.cej.2022.137085 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Park, Sohyun
Su Jeon, Chang
Choi, Namhyun
Moon, Joung-Il
Min Lee, Kang
Hyun Pyun, Sung
Kang, Taejoon
Choo, Jaebum
Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
title Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
title_full Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
title_fullStr Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
title_full_unstemmed Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
title_short Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
title_sort sensitive and reproducible detection of sars-cov-2 using sers-based microdroplet sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121656/
https://www.ncbi.nlm.nih.gov/pubmed/35611066
http://dx.doi.org/10.1016/j.cej.2022.137085
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