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Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor
Surface-enhanced Raman scattering (SERS)-based assays have been recently developed to overcome the low detection sensitivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SERS-based assays using magnetic beads in microtubes slightly improved the limit of detection (LoD) for SARS-C...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121656/ https://www.ncbi.nlm.nih.gov/pubmed/35611066 http://dx.doi.org/10.1016/j.cej.2022.137085 |
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author | Park, Sohyun Su Jeon, Chang Choi, Namhyun Moon, Joung-Il Min Lee, Kang Hyun Pyun, Sung Kang, Taejoon Choo, Jaebum |
author_facet | Park, Sohyun Su Jeon, Chang Choi, Namhyun Moon, Joung-Il Min Lee, Kang Hyun Pyun, Sung Kang, Taejoon Choo, Jaebum |
author_sort | Park, Sohyun |
collection | PubMed |
description | Surface-enhanced Raman scattering (SERS)-based assays have been recently developed to overcome the low detection sensitivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SERS-based assays using magnetic beads in microtubes slightly improved the limit of detection (LoD) for SARS-CoV-2. However, the sensitivity and reproducibility of the method are still insufficient for reliable SARS-CoV-2 detection. In this study, we developed a SERS-based microdroplet sensor to dramatically improve the LoD and reproducibility of SARS-CoV-2 detection. Raman signals were measured for SERS nanotags in 140 droplets passing through a laser focal volume fixed at the center of the channel for 15 s. A comparison of the Raman signals of SERS nanotags measured in a microtube with those measured for multiple droplets in the microfluidic channel revealed that the LoD and coefficient of variation significantly improved from 36 to 0.22 PFU/mL and 21.2% to 1.79%, respectively. This improvement resulted from the ensemble average effects because the signals were measured for SERS nanotags in multiple droplets. Moreover, the total assay time decreased from 30 to 10 min. A clinical test was performed on patient samples to evaluate the clinical efficacy of the SERS-based microdroplet sensor. The assay results agreed well with those measured by the reverse transcription-polymerase chain reaction (RT-PCR) method. The proposed SERS-based microdroplet sensor is expected to be used as a new point-of-care diagnostic platform for quick and accurate detection of SARS-CoV-2 in the field. |
format | Online Article Text |
id | pubmed-9121656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91216562022-05-20 Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor Park, Sohyun Su Jeon, Chang Choi, Namhyun Moon, Joung-Il Min Lee, Kang Hyun Pyun, Sung Kang, Taejoon Choo, Jaebum Chem Eng J Article Surface-enhanced Raman scattering (SERS)-based assays have been recently developed to overcome the low detection sensitivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SERS-based assays using magnetic beads in microtubes slightly improved the limit of detection (LoD) for SARS-CoV-2. However, the sensitivity and reproducibility of the method are still insufficient for reliable SARS-CoV-2 detection. In this study, we developed a SERS-based microdroplet sensor to dramatically improve the LoD and reproducibility of SARS-CoV-2 detection. Raman signals were measured for SERS nanotags in 140 droplets passing through a laser focal volume fixed at the center of the channel for 15 s. A comparison of the Raman signals of SERS nanotags measured in a microtube with those measured for multiple droplets in the microfluidic channel revealed that the LoD and coefficient of variation significantly improved from 36 to 0.22 PFU/mL and 21.2% to 1.79%, respectively. This improvement resulted from the ensemble average effects because the signals were measured for SERS nanotags in multiple droplets. Moreover, the total assay time decreased from 30 to 10 min. A clinical test was performed on patient samples to evaluate the clinical efficacy of the SERS-based microdroplet sensor. The assay results agreed well with those measured by the reverse transcription-polymerase chain reaction (RT-PCR) method. The proposed SERS-based microdroplet sensor is expected to be used as a new point-of-care diagnostic platform for quick and accurate detection of SARS-CoV-2 in the field. Elsevier B.V. 2022-10-15 2022-05-20 /pmc/articles/PMC9121656/ /pubmed/35611066 http://dx.doi.org/10.1016/j.cej.2022.137085 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Park, Sohyun Su Jeon, Chang Choi, Namhyun Moon, Joung-Il Min Lee, Kang Hyun Pyun, Sung Kang, Taejoon Choo, Jaebum Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor |
title | Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor |
title_full | Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor |
title_fullStr | Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor |
title_full_unstemmed | Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor |
title_short | Sensitive and reproducible detection of SARS-CoV-2 using SERS-based microdroplet sensor |
title_sort | sensitive and reproducible detection of sars-cov-2 using sers-based microdroplet sensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121656/ https://www.ncbi.nlm.nih.gov/pubmed/35611066 http://dx.doi.org/10.1016/j.cej.2022.137085 |
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