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T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles

CD8(+) T cells are a pivotal part of the immune response to viruses, playing a key role in disease outcome and providing long-lasting immunity to conserved pathogen epitopes. Understanding CD8(+) T cell immunity in humans is complex due to CD8(+) T cell restriction by highly polymorphic Human Leukoc...

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Autores principales: Hensen, Luca, Illing, Patricia T., Rowntree, Louise C., Davies, Jane, Miller, Adrian, Tong, Steven Y. C., Habel, Jennifer R., van de Sandt, Carolien E., Flanagan, Katie L., Purcell, Anthony W., Kedzierska, Katherine, Clemens, E. Bridie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121770/
https://www.ncbi.nlm.nih.gov/pubmed/35603215
http://dx.doi.org/10.3389/fimmu.2022.812393
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author Hensen, Luca
Illing, Patricia T.
Rowntree, Louise C.
Davies, Jane
Miller, Adrian
Tong, Steven Y. C.
Habel, Jennifer R.
van de Sandt, Carolien E.
Flanagan, Katie L.
Purcell, Anthony W.
Kedzierska, Katherine
Clemens, E. Bridie
author_facet Hensen, Luca
Illing, Patricia T.
Rowntree, Louise C.
Davies, Jane
Miller, Adrian
Tong, Steven Y. C.
Habel, Jennifer R.
van de Sandt, Carolien E.
Flanagan, Katie L.
Purcell, Anthony W.
Kedzierska, Katherine
Clemens, E. Bridie
author_sort Hensen, Luca
collection PubMed
description CD8(+) T cells are a pivotal part of the immune response to viruses, playing a key role in disease outcome and providing long-lasting immunity to conserved pathogen epitopes. Understanding CD8(+) T cell immunity in humans is complex due to CD8(+) T cell restriction by highly polymorphic Human Leukocyte Antigen (HLA) proteins, requiring T cell epitopes to be defined for different HLA allotypes across different ethnicities. Here we evaluate strategies that have been developed to facilitate epitope identification and study immunogenic T cell responses. We describe an immunopeptidomics approach to sequence HLA-bound peptides presented on virus-infected cells by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Using antigen presenting cell lines that stably express the HLA alleles characteristic of Indigenous Australians, this approach has been successfully used to comprehensively identify influenza-specific CD8(+) T cell epitopes restricted by HLA allotypes predominant in Indigenous Australians, including HLA-A*24:02 and HLA-A*11:01. This is an essential step in ensuring high vaccine coverage and efficacy in Indigenous populations globally, known to be at high risk from influenza disease and other respiratory infections.
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spelling pubmed-91217702022-05-21 T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles Hensen, Luca Illing, Patricia T. Rowntree, Louise C. Davies, Jane Miller, Adrian Tong, Steven Y. C. Habel, Jennifer R. van de Sandt, Carolien E. Flanagan, Katie L. Purcell, Anthony W. Kedzierska, Katherine Clemens, E. Bridie Front Immunol Immunology CD8(+) T cells are a pivotal part of the immune response to viruses, playing a key role in disease outcome and providing long-lasting immunity to conserved pathogen epitopes. Understanding CD8(+) T cell immunity in humans is complex due to CD8(+) T cell restriction by highly polymorphic Human Leukocyte Antigen (HLA) proteins, requiring T cell epitopes to be defined for different HLA allotypes across different ethnicities. Here we evaluate strategies that have been developed to facilitate epitope identification and study immunogenic T cell responses. We describe an immunopeptidomics approach to sequence HLA-bound peptides presented on virus-infected cells by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Using antigen presenting cell lines that stably express the HLA alleles characteristic of Indigenous Australians, this approach has been successfully used to comprehensively identify influenza-specific CD8(+) T cell epitopes restricted by HLA allotypes predominant in Indigenous Australians, including HLA-A*24:02 and HLA-A*11:01. This is an essential step in ensuring high vaccine coverage and efficacy in Indigenous populations globally, known to be at high risk from influenza disease and other respiratory infections. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9121770/ /pubmed/35603215 http://dx.doi.org/10.3389/fimmu.2022.812393 Text en Copyright © 2022 Hensen, Illing, Rowntree, Davies, Miller, Tong, Habel, van de Sandt, Flanagan, Purcell, Kedzierska and Clemens https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hensen, Luca
Illing, Patricia T.
Rowntree, Louise C.
Davies, Jane
Miller, Adrian
Tong, Steven Y. C.
Habel, Jennifer R.
van de Sandt, Carolien E.
Flanagan, Katie L.
Purcell, Anthony W.
Kedzierska, Katherine
Clemens, E. Bridie
T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles
title T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles
title_full T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles
title_fullStr T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles
title_full_unstemmed T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles
title_short T Cell Epitope Discovery in the Context of Distinct and Unique Indigenous HLA Profiles
title_sort t cell epitope discovery in the context of distinct and unique indigenous hla profiles
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121770/
https://www.ncbi.nlm.nih.gov/pubmed/35603215
http://dx.doi.org/10.3389/fimmu.2022.812393
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