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Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya

INTRODUCTION: Current diagnostic tools for schistosomiasis are limited, and new tests are necessary to enhance disease diagnosis and surveillance. Identification of novel disease-specific biomarkers may facilitate the development of such tests. We evaluated a panel of biomarkers used in sepsis and p...

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Autores principales: Ondigo, Bartholomew N., Hamilton, Rachael E., Magomere, Edwin O., Onkanga, Isaac O., Mwinzi, Pauline N., Odiere, Maurice R., Ganley-Leal, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121796/
https://www.ncbi.nlm.nih.gov/pubmed/35603171
http://dx.doi.org/10.3389/fimmu.2022.887213
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author Ondigo, Bartholomew N.
Hamilton, Rachael E.
Magomere, Edwin O.
Onkanga, Isaac O.
Mwinzi, Pauline N.
Odiere, Maurice R.
Ganley-Leal, Lisa
author_facet Ondigo, Bartholomew N.
Hamilton, Rachael E.
Magomere, Edwin O.
Onkanga, Isaac O.
Mwinzi, Pauline N.
Odiere, Maurice R.
Ganley-Leal, Lisa
author_sort Ondigo, Bartholomew N.
collection PubMed
description INTRODUCTION: Current diagnostic tools for schistosomiasis are limited, and new tests are necessary to enhance disease diagnosis and surveillance. Identification of novel disease-specific biomarkers may facilitate the development of such tests. We evaluated a panel of biomarkers used in sepsis and parasitic diseases for their potential suitability in the diagnosis of schistosomiasis. OBJECTIVE: The study evaluated the levels of systemic plasma biomarkers in relation to Schistosoma mansoni infection and parasite burden. METHODS: Six biomarkers were measured in the plasma of children from schistosomiasis-endemic regions using ELISA. The concentration of soluble CD23 (sCD23) and lipopolysaccharide (LPS) was tested in 199 and 124 plasma samples, respectively, while interleukin-6 (IL-6), soluble triggering receptor expressed on myeloid (sTREM) cells, eotaxin-1, and fatty acid-binding protein (FABP) concentrations were tested in 30 plasma samples. RESULTS: The concentration of IL-6, eotaxin-1, FABP, and LPS was similar between schistosome-infected and uninfected children. The schistosome-infected children had higher median levels of sTREM and sCD23 as compared to uninfected children, 119.0 (29.9–208.9) versus 10.7 (0.0–73.4) (p = 0.046) and 2,549.0 (1,899.0–3,356.0) vs. 2,035.0 (1,448.0–2,939.0) (p = 0.05), respectively. In addition, sTREM was positively correlated with egg density (p = 0.017). CONCLUSION: Our data show that active schistosomiasis per se is associated with elevated levels of sTREM and sCD23. sTREM has potential diagnostic and prognostic values. However, these biomarkers did not distinguish between children with low egg burden and uninfected children.
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spelling pubmed-91217962022-05-21 Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya Ondigo, Bartholomew N. Hamilton, Rachael E. Magomere, Edwin O. Onkanga, Isaac O. Mwinzi, Pauline N. Odiere, Maurice R. Ganley-Leal, Lisa Front Immunol Immunology INTRODUCTION: Current diagnostic tools for schistosomiasis are limited, and new tests are necessary to enhance disease diagnosis and surveillance. Identification of novel disease-specific biomarkers may facilitate the development of such tests. We evaluated a panel of biomarkers used in sepsis and parasitic diseases for their potential suitability in the diagnosis of schistosomiasis. OBJECTIVE: The study evaluated the levels of systemic plasma biomarkers in relation to Schistosoma mansoni infection and parasite burden. METHODS: Six biomarkers were measured in the plasma of children from schistosomiasis-endemic regions using ELISA. The concentration of soluble CD23 (sCD23) and lipopolysaccharide (LPS) was tested in 199 and 124 plasma samples, respectively, while interleukin-6 (IL-6), soluble triggering receptor expressed on myeloid (sTREM) cells, eotaxin-1, and fatty acid-binding protein (FABP) concentrations were tested in 30 plasma samples. RESULTS: The concentration of IL-6, eotaxin-1, FABP, and LPS was similar between schistosome-infected and uninfected children. The schistosome-infected children had higher median levels of sTREM and sCD23 as compared to uninfected children, 119.0 (29.9–208.9) versus 10.7 (0.0–73.4) (p = 0.046) and 2,549.0 (1,899.0–3,356.0) vs. 2,035.0 (1,448.0–2,939.0) (p = 0.05), respectively. In addition, sTREM was positively correlated with egg density (p = 0.017). CONCLUSION: Our data show that active schistosomiasis per se is associated with elevated levels of sTREM and sCD23. sTREM has potential diagnostic and prognostic values. However, these biomarkers did not distinguish between children with low egg burden and uninfected children. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9121796/ /pubmed/35603171 http://dx.doi.org/10.3389/fimmu.2022.887213 Text en Copyright © 2022 Ondigo, Hamilton, Magomere, Onkanga, Mwinzi, Odiere and Ganley-Leal https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ondigo, Bartholomew N.
Hamilton, Rachael E.
Magomere, Edwin O.
Onkanga, Isaac O.
Mwinzi, Pauline N.
Odiere, Maurice R.
Ganley-Leal, Lisa
Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
title Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
title_full Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
title_fullStr Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
title_full_unstemmed Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
title_short Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
title_sort potential utility of systemic plasma biomarkers for evaluation of pediatric schistosomiasis in western kenya
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121796/
https://www.ncbi.nlm.nih.gov/pubmed/35603171
http://dx.doi.org/10.3389/fimmu.2022.887213
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