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Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S
Endoplasmic reticulum (ER) stress, which can be induced by reactive oxygen species (ROS) and multiple factors, is associated with numerous intestinal diseases. The organic selenium source 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), has been proved to decrease intestinal inflammation and autophag...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121856/ https://www.ncbi.nlm.nih.gov/pubmed/35600832 http://dx.doi.org/10.3389/fnut.2022.900421 |
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author | Ding, Dajiang Mou, Daolin Zhu, Heng Jiang, Xuemei Che, Lianqiang Fang, Zhengfeng Xu, Shengyu Lin, Yan Zhuo, Yong Li, Jian Huang, Chao Zou, Yuanfeng Li, Lixia Wu, De Feng, Bin |
author_facet | Ding, Dajiang Mou, Daolin Zhu, Heng Jiang, Xuemei Che, Lianqiang Fang, Zhengfeng Xu, Shengyu Lin, Yan Zhuo, Yong Li, Jian Huang, Chao Zou, Yuanfeng Li, Lixia Wu, De Feng, Bin |
author_sort | Ding, Dajiang |
collection | PubMed |
description | Endoplasmic reticulum (ER) stress, which can be induced by reactive oxygen species (ROS) and multiple factors, is associated with numerous intestinal diseases. The organic selenium source 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), has been proved to decrease intestinal inflammation and autophagy by improving the expression of selenoproteins. However, it remains unclear whether HMSeBA could alleviate intestinal ER stress by decreasing excessive production of ROS products. This study was conducted to investigate the effect of maternal HMSeBA supplementation on the regulation of intestinal ER stress of their offspring and the regulatory mechanism. Sows were supplemented with HMSeBA during gestation and jejunal epithelial (IPEC-J2) cells were treatment with HMSeBA. Results showed that maternal HMSeBA supplementation significantly upregulated mRNA level of selenoprotein S (SELS) in the jejunum of newborn and weaned piglets compared with the control group, while decreased the gene expression and protein abundance of ER stress markers in the jejunum of LPS challenged weaned piglets. In addition, HMSeBA treatment significantly increased the expression of glutathione peroxidase 4 (GPX4) and SELS, while decreased ROS level and the expression of ER stress markers induced by hydrogen peroxide (H(2)O(2)) in IPEC-J2 cells. Furthermore, knockdown of GPX4 did not enhance the ERS signal induced by H(2)O(2), but the lack of GPX4 would cause further deterioration of ER stress signal in the absence of SELS. In conclusion, maternal HMSeBA supplementation might alleviate ROS induced intestinal ER stress by improving the expression of SELS and GPX4 in their offspring. Thus, maternal HMSeBA supplementation might be benefit for the intestinal health of their offspring. |
format | Online Article Text |
id | pubmed-9121856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91218562022-05-21 Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S Ding, Dajiang Mou, Daolin Zhu, Heng Jiang, Xuemei Che, Lianqiang Fang, Zhengfeng Xu, Shengyu Lin, Yan Zhuo, Yong Li, Jian Huang, Chao Zou, Yuanfeng Li, Lixia Wu, De Feng, Bin Front Nutr Nutrition Endoplasmic reticulum (ER) stress, which can be induced by reactive oxygen species (ROS) and multiple factors, is associated with numerous intestinal diseases. The organic selenium source 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), has been proved to decrease intestinal inflammation and autophagy by improving the expression of selenoproteins. However, it remains unclear whether HMSeBA could alleviate intestinal ER stress by decreasing excessive production of ROS products. This study was conducted to investigate the effect of maternal HMSeBA supplementation on the regulation of intestinal ER stress of their offspring and the regulatory mechanism. Sows were supplemented with HMSeBA during gestation and jejunal epithelial (IPEC-J2) cells were treatment with HMSeBA. Results showed that maternal HMSeBA supplementation significantly upregulated mRNA level of selenoprotein S (SELS) in the jejunum of newborn and weaned piglets compared with the control group, while decreased the gene expression and protein abundance of ER stress markers in the jejunum of LPS challenged weaned piglets. In addition, HMSeBA treatment significantly increased the expression of glutathione peroxidase 4 (GPX4) and SELS, while decreased ROS level and the expression of ER stress markers induced by hydrogen peroxide (H(2)O(2)) in IPEC-J2 cells. Furthermore, knockdown of GPX4 did not enhance the ERS signal induced by H(2)O(2), but the lack of GPX4 would cause further deterioration of ER stress signal in the absence of SELS. In conclusion, maternal HMSeBA supplementation might alleviate ROS induced intestinal ER stress by improving the expression of SELS and GPX4 in their offspring. Thus, maternal HMSeBA supplementation might be benefit for the intestinal health of their offspring. Frontiers Media S.A. 2022-05-06 /pmc/articles/PMC9121856/ /pubmed/35600832 http://dx.doi.org/10.3389/fnut.2022.900421 Text en Copyright © 2022 Ding, Mou, Zhu, Jiang, Che, Fang, Xu, Lin, Zhuo, Li, Huang, Zou, Li, Wu and Feng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Ding, Dajiang Mou, Daolin Zhu, Heng Jiang, Xuemei Che, Lianqiang Fang, Zhengfeng Xu, Shengyu Lin, Yan Zhuo, Yong Li, Jian Huang, Chao Zou, Yuanfeng Li, Lixia Wu, De Feng, Bin Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
title | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
title_full | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
title_fullStr | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
title_full_unstemmed | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
title_short | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
title_sort | maternal organic selenium supplementation relieves intestinal endoplasmic reticulum stress in piglets by enhancing the expression of glutathione peroxidase 4 and selenoprotein s |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121856/ https://www.ncbi.nlm.nih.gov/pubmed/35600832 http://dx.doi.org/10.3389/fnut.2022.900421 |
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