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The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats

OBJECTIVES: Methionine is known as an essential amino acid in mammals. Consuming excessive amounts of methionine has toxic effects. This study aimed at evaluating the histomorphometric and histopathologic changes of ovaries after methionine administration during follicle formation. MATERIAL AND METH...

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Autores principales: Nazem, Mohamad Naser, Aghamiri, Seyed Morteza, Kheirandish, Reza, Hakimy, Zeinab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122406/
https://www.ncbi.nlm.nih.gov/pubmed/35133706
http://dx.doi.org/10.1002/vms3.750
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author Nazem, Mohamad Naser
Aghamiri, Seyed Morteza
Kheirandish, Reza
Hakimy, Zeinab
author_facet Nazem, Mohamad Naser
Aghamiri, Seyed Morteza
Kheirandish, Reza
Hakimy, Zeinab
author_sort Nazem, Mohamad Naser
collection PubMed
description OBJECTIVES: Methionine is known as an essential amino acid in mammals. Consuming excessive amounts of methionine has toxic effects. This study aimed at evaluating the histomorphometric and histopathologic changes of ovaries after methionine administration during follicle formation. MATERIAL AND METHODS: A total of 60 newborn female rats born under similar conditions were selected and randomly assigned into three groups including control, recipients of 50 and 200 mg/kg body weight of methionine for 5 days. On day 120, all 60 female rats were euthanized and the whole left ovary of each animal was taken in order to count the number of primordial, primary, secondary, antral, atretic follicles, as well as corpora lutea and also to conduct histopathologic study. RESULTS: According to the results, the 50 mg/kg methionine did not significantly change the number of primordial follicles compared to the control group but the 200 mg/kg dose significantly decreased the number of primordial follicles. There were no significant differences between the groups in the number of other types of follicles and also in the number of corpora lutea. There was no histopathological lesion in the groups. CONCLUSIONS: It seems that the high dose of methionine could exacerbate apoptosis of the primordial ovarian follicle during the follicle assembly process. However, the remaining were enough to form later stages of follicles after puberty.
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spelling pubmed-91224062022-05-21 The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats Nazem, Mohamad Naser Aghamiri, Seyed Morteza Kheirandish, Reza Hakimy, Zeinab Vet Med Sci RODENTS OBJECTIVES: Methionine is known as an essential amino acid in mammals. Consuming excessive amounts of methionine has toxic effects. This study aimed at evaluating the histomorphometric and histopathologic changes of ovaries after methionine administration during follicle formation. MATERIAL AND METHODS: A total of 60 newborn female rats born under similar conditions were selected and randomly assigned into three groups including control, recipients of 50 and 200 mg/kg body weight of methionine for 5 days. On day 120, all 60 female rats were euthanized and the whole left ovary of each animal was taken in order to count the number of primordial, primary, secondary, antral, atretic follicles, as well as corpora lutea and also to conduct histopathologic study. RESULTS: According to the results, the 50 mg/kg methionine did not significantly change the number of primordial follicles compared to the control group but the 200 mg/kg dose significantly decreased the number of primordial follicles. There were no significant differences between the groups in the number of other types of follicles and also in the number of corpora lutea. There was no histopathological lesion in the groups. CONCLUSIONS: It seems that the high dose of methionine could exacerbate apoptosis of the primordial ovarian follicle during the follicle assembly process. However, the remaining were enough to form later stages of follicles after puberty. John Wiley and Sons Inc. 2022-02-08 /pmc/articles/PMC9122406/ /pubmed/35133706 http://dx.doi.org/10.1002/vms3.750 Text en © 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle RODENTS
Nazem, Mohamad Naser
Aghamiri, Seyed Morteza
Kheirandish, Reza
Hakimy, Zeinab
The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
title The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
title_full The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
title_fullStr The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
title_full_unstemmed The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
title_short The effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
title_sort effects of methionine administration during the beginning postnatal days on the ovarian structures in adult rats
topic RODENTS
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122406/
https://www.ncbi.nlm.nih.gov/pubmed/35133706
http://dx.doi.org/10.1002/vms3.750
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