Cargando…

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

The fabrication of biomimetic structures for tissues and organs is emerging in the fields of biomedical engineering and precision medicine. While current progress in biomedical research provides a number of biofabrication methods, the construction of multi-dimensional cardiac tissue is highly challe...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, Yongjun, Jung, Da Jung, Choi, Seung-Cheol, Lim, Do-Sun, Kim, Jong-Hoon, Jeoung, Gi Seok, Kim, Jongseong, Park, Yongdoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122566/
https://www.ncbi.nlm.nih.gov/pubmed/35693693
http://dx.doi.org/10.1039/d0ra01577f
_version_ 1784711372079104000
author Jang, Yongjun
Jung, Da Jung
Choi, Seung-Cheol
Lim, Do-Sun
Kim, Jong-Hoon
Jeoung, Gi Seok
Kim, Jongseong
Park, Yongdoo
author_facet Jang, Yongjun
Jung, Da Jung
Choi, Seung-Cheol
Lim, Do-Sun
Kim, Jong-Hoon
Jeoung, Gi Seok
Kim, Jongseong
Park, Yongdoo
author_sort Jang, Yongjun
collection PubMed
description The fabrication of biomimetic structures for tissues and organs is emerging in the fields of biomedical engineering and precision medicine. While current progress in biomedical research provides a number of biofabrication methods, the construction of multi-dimensional cardiac tissue is highly challenging due to difficulties in the maturation and synchronization of cardiomyocytes (CMs) in conjunction with other types of cells, such as myofibroblasts and endothelial cells. Here, we show a simple fabrication methodology to construct multi-dimensional cardiac macro tissue (mCMT) by layer-by-layer (LBL) deposition of cells on micro patterned PDMS. mCMTs formed by LBL deposition of pluripotent stem cell (PSC)-derived cardiomyocytes and cardiac fibroblasts formed 3D patterned structures with synchronized beating characteristics. We also demonstrate that cardiac maturation factors such as the gene expression of MLC2v and cTNI and formation of sarcomeres in mCMTs were significantly enhanced by LBL deposition and growth factors during the maturation process. Fabrication of matured mCMTs with synchronized beating enables providing an efficient platform for evaluating the efficacy and toxicity of drug candidates. These results have important implications because mCMTs are applicable to diverse in vitro studies and drug screening methods that require tissue-like structures and functions in a physiological environment.
format Online
Article
Text
id pubmed-9122566
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-91225662022-06-10 Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues Jang, Yongjun Jung, Da Jung Choi, Seung-Cheol Lim, Do-Sun Kim, Jong-Hoon Jeoung, Gi Seok Kim, Jongseong Park, Yongdoo RSC Adv Chemistry The fabrication of biomimetic structures for tissues and organs is emerging in the fields of biomedical engineering and precision medicine. While current progress in biomedical research provides a number of biofabrication methods, the construction of multi-dimensional cardiac tissue is highly challenging due to difficulties in the maturation and synchronization of cardiomyocytes (CMs) in conjunction with other types of cells, such as myofibroblasts and endothelial cells. Here, we show a simple fabrication methodology to construct multi-dimensional cardiac macro tissue (mCMT) by layer-by-layer (LBL) deposition of cells on micro patterned PDMS. mCMTs formed by LBL deposition of pluripotent stem cell (PSC)-derived cardiomyocytes and cardiac fibroblasts formed 3D patterned structures with synchronized beating characteristics. We also demonstrate that cardiac maturation factors such as the gene expression of MLC2v and cTNI and formation of sarcomeres in mCMTs were significantly enhanced by LBL deposition and growth factors during the maturation process. Fabrication of matured mCMTs with synchronized beating enables providing an efficient platform for evaluating the efficacy and toxicity of drug candidates. These results have important implications because mCMTs are applicable to diverse in vitro studies and drug screening methods that require tissue-like structures and functions in a physiological environment. The Royal Society of Chemistry 2020-05-18 /pmc/articles/PMC9122566/ /pubmed/35693693 http://dx.doi.org/10.1039/d0ra01577f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Jang, Yongjun
Jung, Da Jung
Choi, Seung-Cheol
Lim, Do-Sun
Kim, Jong-Hoon
Jeoung, Gi Seok
Kim, Jongseong
Park, Yongdoo
Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
title Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
title_full Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
title_fullStr Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
title_full_unstemmed Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
title_short Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
title_sort multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122566/
https://www.ncbi.nlm.nih.gov/pubmed/35693693
http://dx.doi.org/10.1039/d0ra01577f
work_keys_str_mv AT jangyongjun multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT jungdajung multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT choiseungcheol multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT limdosun multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT kimjonghoon multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT jeounggiseok multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT kimjongseong multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues
AT parkyongdoo multidimensionalassemblyusinglayerbylayerdepositionforsynchronizedcardiacmacrotissues