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Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery

Barcode fusion genetics (BFG) utilizes deep sequencing to improve the throughput of protein–protein interaction (PPI) screening in pools. BFG has been implemented in Yeast two-hybrid (Y2H) screens (BFG-Y2H). While Y2H requires test protein pairs to localize in the nucleus for reporter reconstruction...

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Detalles Bibliográficos
Autores principales:	Evans-Yamamoto, Daniel, Rouleau, François D, Nanda, Piyush, Makanae, Koji, Liu, Yin, Després, Philippe C, Matsuo, Hitoshi, Seki, Motoaki, Dubé, Alexandre K, Ascencio, Diana, Yachie, Nozomu, Landry, Christian R
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122585/ https://www.ncbi.nlm.nih.gov/pubmed/35137167 http://dx.doi.org/10.1093/nar/gkac045

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