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Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation
Extracellular vesicles (EVs) are single‐membrane vesicles that play an essential role in long‐range intercellular communications. EV investigation has been explored largely through cell‐culture systems, but it remains unclear how physiological EVs exert homeostatic or pathological functions in vivo....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122834/ https://www.ncbi.nlm.nih.gov/pubmed/35595717 http://dx.doi.org/10.1002/jev2.12223 |
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author | Liu, Bowen Jin, Yuan Yang, Jingyi Han, Yue Shan, Hui Qiu, Mantang Zhao, Xuyang Liu, Anhang Jin, Yan Yin, Yuxin |
author_facet | Liu, Bowen Jin, Yuan Yang, Jingyi Han, Yue Shan, Hui Qiu, Mantang Zhao, Xuyang Liu, Anhang Jin, Yan Yin, Yuxin |
author_sort | Liu, Bowen |
collection | PubMed |
description | Extracellular vesicles (EVs) are single‐membrane vesicles that play an essential role in long‐range intercellular communications. EV investigation has been explored largely through cell‐culture systems, but it remains unclear how physiological EVs exert homeostatic or pathological functions in vivo. Here, we report that lung EVs promote chemotaxis of neutrophils in bone marrow through delivery of double stranded DNA (dsDNA). We have identified and characterized EVs containing dsDNA collected from both human and murine lung tissues using newly developed approaches. Our analysis of EV proteomics together with single‐cell RNA sequencing data reveals that type II alveolar epithelial cells are the main source of the lung EVs. Furthermore, we demonstrate that the lung EVs accumulate in bone marrow and enhance neutrophil recruitment under inflammation conditions. Moreover, lung EV‐DNA stimulates neutrophils to release the chemokines CXCL1 and CXCL2 via DNA‐TLR9 signalling. Our findings establish a molecular basis of lung EVs in enhancement of host immune response to bacterial infection and provide new insights into understanding of vesicle‐mediated systematic communications. |
format | Online Article Text |
id | pubmed-9122834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-91228342022-05-21 Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation Liu, Bowen Jin, Yuan Yang, Jingyi Han, Yue Shan, Hui Qiu, Mantang Zhao, Xuyang Liu, Anhang Jin, Yan Yin, Yuxin J Extracell Vesicles Research Articles Extracellular vesicles (EVs) are single‐membrane vesicles that play an essential role in long‐range intercellular communications. EV investigation has been explored largely through cell‐culture systems, but it remains unclear how physiological EVs exert homeostatic or pathological functions in vivo. Here, we report that lung EVs promote chemotaxis of neutrophils in bone marrow through delivery of double stranded DNA (dsDNA). We have identified and characterized EVs containing dsDNA collected from both human and murine lung tissues using newly developed approaches. Our analysis of EV proteomics together with single‐cell RNA sequencing data reveals that type II alveolar epithelial cells are the main source of the lung EVs. Furthermore, we demonstrate that the lung EVs accumulate in bone marrow and enhance neutrophil recruitment under inflammation conditions. Moreover, lung EV‐DNA stimulates neutrophils to release the chemokines CXCL1 and CXCL2 via DNA‐TLR9 signalling. Our findings establish a molecular basis of lung EVs in enhancement of host immune response to bacterial infection and provide new insights into understanding of vesicle‐mediated systematic communications. John Wiley and Sons Inc. 2022-05-20 2022-05 /pmc/articles/PMC9122834/ /pubmed/35595717 http://dx.doi.org/10.1002/jev2.12223 Text en © 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Liu, Bowen Jin, Yuan Yang, Jingyi Han, Yue Shan, Hui Qiu, Mantang Zhao, Xuyang Liu, Anhang Jin, Yan Yin, Yuxin Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
title | Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
title_full | Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
title_fullStr | Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
title_full_unstemmed | Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
title_short | Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
title_sort | extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122834/ https://www.ncbi.nlm.nih.gov/pubmed/35595717 http://dx.doi.org/10.1002/jev2.12223 |
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