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Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus
Human noroviruses (hNoVs) are an important cause of acute gastroenteritis worldwide. However, the lack of a reproducible in vitro cell culture system has impaired research and the development of preventive measures, therapeutic drugs, and vaccines. The aim of this study was to analyze and optimize a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123034/ https://www.ncbi.nlm.nih.gov/pubmed/35415782 http://dx.doi.org/10.1007/s00705-022-05437-3 |
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author | Pohl, Clara Szczepankiewicz, Grit Liebert, Uwe Gerd |
author_facet | Pohl, Clara Szczepankiewicz, Grit Liebert, Uwe Gerd |
author_sort | Pohl, Clara |
collection | PubMed |
description | Human noroviruses (hNoVs) are an important cause of acute gastroenteritis worldwide. However, the lack of a reproducible in vitro cell culture system has impaired research and the development of preventive measures, therapeutic drugs, and vaccines. The aim of this study was to analyze and optimize a suitable cell line for in vitro cultivation of hNoV. The Caco-2 cell line, which is of colorectal origin and differentiates spontaneously into intestinal enterocyte-like cells, was chosen as a model. It was found that differentiated cells were more susceptible to infection with hNoV, resulting in a higher virus yield. This was accompanied by an increase in H type 1 antigen in the cell membrane during differentiation, which functions as an attachment factor for hNoV. Induced overexpression of H type 1 antigen in undifferentiated Caco-2 cells resulted in an increase in viral output to a level similar to that in differentiated cells. However, the relatively low level of viral output, which contrasts with what is observed in vivo, shows that the viral replication cycle is restricted in this model. The results indicate that there is a block at the level of viral release. |
format | Online Article Text |
id | pubmed-9123034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-91230342022-05-22 Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus Pohl, Clara Szczepankiewicz, Grit Liebert, Uwe Gerd Arch Virol Original Article Human noroviruses (hNoVs) are an important cause of acute gastroenteritis worldwide. However, the lack of a reproducible in vitro cell culture system has impaired research and the development of preventive measures, therapeutic drugs, and vaccines. The aim of this study was to analyze and optimize a suitable cell line for in vitro cultivation of hNoV. The Caco-2 cell line, which is of colorectal origin and differentiates spontaneously into intestinal enterocyte-like cells, was chosen as a model. It was found that differentiated cells were more susceptible to infection with hNoV, resulting in a higher virus yield. This was accompanied by an increase in H type 1 antigen in the cell membrane during differentiation, which functions as an attachment factor for hNoV. Induced overexpression of H type 1 antigen in undifferentiated Caco-2 cells resulted in an increase in viral output to a level similar to that in differentiated cells. However, the relatively low level of viral output, which contrasts with what is observed in vivo, shows that the viral replication cycle is restricted in this model. The results indicate that there is a block at the level of viral release. Springer Vienna 2022-04-12 2022 /pmc/articles/PMC9123034/ /pubmed/35415782 http://dx.doi.org/10.1007/s00705-022-05437-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Pohl, Clara Szczepankiewicz, Grit Liebert, Uwe Gerd Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus |
title | Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus |
title_full | Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus |
title_fullStr | Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus |
title_full_unstemmed | Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus |
title_short | Analysis and optimization of a Caco-2 cell culture model for infection with human norovirus |
title_sort | analysis and optimization of a caco-2 cell culture model for infection with human norovirus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123034/ https://www.ncbi.nlm.nih.gov/pubmed/35415782 http://dx.doi.org/10.1007/s00705-022-05437-3 |
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