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Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface

Healing of an anterior cruciate ligament (ACL) autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue, which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failu...

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Autores principales: Jiang, Qingsong, Wang, Liren, Liu, Zhanhong, Su, Jinlei, Tang, Yajun, Tan, Peijie, Zhu, Xiangdong, Zhang, Kai, Ma, Xing, Jiang, Jia, Zhao, Jinzhong, Lin, Hai, Zhang, Xingdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123091/
https://www.ncbi.nlm.nih.gov/pubmed/35633878
http://dx.doi.org/10.1016/j.bioactmat.2022.05.003
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author Jiang, Qingsong
Wang, Liren
Liu, Zhanhong
Su, Jinlei
Tang, Yajun
Tan, Peijie
Zhu, Xiangdong
Zhang, Kai
Ma, Xing
Jiang, Jia
Zhao, Jinzhong
Lin, Hai
Zhang, Xingdong
author_facet Jiang, Qingsong
Wang, Liren
Liu, Zhanhong
Su, Jinlei
Tang, Yajun
Tan, Peijie
Zhu, Xiangdong
Zhang, Kai
Ma, Xing
Jiang, Jia
Zhao, Jinzhong
Lin, Hai
Zhang, Xingdong
author_sort Jiang, Qingsong
collection PubMed
description Healing of an anterior cruciate ligament (ACL) autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue, which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence. In this study, an injectable hydroxyapatite/type I collagen (HAp/Col Ⅰ) paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface. Physicochemical characterization demonstrated that the HAp/Col Ⅰ paste was injectable, uniform and stable. The in vitro cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression. The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL. The average width of the tunnel, total bone volume, bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group. The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL's maximal load at 24 weeks. According to histological examination, Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group. The injectable HAp/Col Ⅰ paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation, further osteogenic expression and extracellular matrix deposition, and in vivo structural and functional regeneration of the tendon-bone interface.
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spelling pubmed-91230912022-05-26 Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface Jiang, Qingsong Wang, Liren Liu, Zhanhong Su, Jinlei Tang, Yajun Tan, Peijie Zhu, Xiangdong Zhang, Kai Ma, Xing Jiang, Jia Zhao, Jinzhong Lin, Hai Zhang, Xingdong Bioact Mater Article Healing of an anterior cruciate ligament (ACL) autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue, which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence. In this study, an injectable hydroxyapatite/type I collagen (HAp/Col Ⅰ) paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface. Physicochemical characterization demonstrated that the HAp/Col Ⅰ paste was injectable, uniform and stable. The in vitro cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression. The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL. The average width of the tunnel, total bone volume, bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group. The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL's maximal load at 24 weeks. According to histological examination, Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group. The injectable HAp/Col Ⅰ paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation, further osteogenic expression and extracellular matrix deposition, and in vivo structural and functional regeneration of the tendon-bone interface. KeAi Publishing 2022-05-17 /pmc/articles/PMC9123091/ /pubmed/35633878 http://dx.doi.org/10.1016/j.bioactmat.2022.05.003 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Jiang, Qingsong
Wang, Liren
Liu, Zhanhong
Su, Jinlei
Tang, Yajun
Tan, Peijie
Zhu, Xiangdong
Zhang, Kai
Ma, Xing
Jiang, Jia
Zhao, Jinzhong
Lin, Hai
Zhang, Xingdong
Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
title Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
title_full Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
title_fullStr Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
title_full_unstemmed Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
title_short Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
title_sort canine acl reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123091/
https://www.ncbi.nlm.nih.gov/pubmed/35633878
http://dx.doi.org/10.1016/j.bioactmat.2022.05.003
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