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D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or...

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Autores principales: Imerb, Napatsorn, Thonusin, Chanisa, Pratchayasakul, Wasana, Arunsak, Busarin, Nawara, Wichwara, Ongnok, Benjamin, Aeimlapa, Ratchaneevan, Charoenphandhu, Narattaphol, Chattipakorn, Nipon, Chattipakorn, Siriporn C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123171/
https://www.ncbi.nlm.nih.gov/pubmed/35595806
http://dx.doi.org/10.1038/s41598-022-12206-4
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author Imerb, Napatsorn
Thonusin, Chanisa
Pratchayasakul, Wasana
Arunsak, Busarin
Nawara, Wichwara
Ongnok, Benjamin
Aeimlapa, Ratchaneevan
Charoenphandhu, Narattaphol
Chattipakorn, Nipon
Chattipakorn, Siriporn C.
author_facet Imerb, Napatsorn
Thonusin, Chanisa
Pratchayasakul, Wasana
Arunsak, Busarin
Nawara, Wichwara
Ongnok, Benjamin
Aeimlapa, Ratchaneevan
Charoenphandhu, Narattaphol
Chattipakorn, Nipon
Chattipakorn, Siriporn C.
author_sort Imerb, Napatsorn
collection PubMed
description We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or 150 mg/kg/day of D-gal for 4 or 8 weeks. Blood was collected to measure metabolic, aging, oxidative stress, and bone turnover parameters. Bone oxidative stress and inflammatory markers, as well as bone histomorphometry were also evaluated. Additionally, RAW 264.7 cells were incubated with either D-gal, insulin, or D-gal plus insulin to identify osteoclast differentiation capacity under the stimulation of receptor activator of nuclear factor κB ligand. At week 4, D-gal-induced aging significantly elevated serum malondialdehyde level and decreased trabecular thickness in ND- and HFD-fed rats, when compared to the control group. At week 8, D-gal-induced aging further elevated advanced glycation end products, increased bone inflammation and resorption, and significantly impaired bone microarchitecture in HFD-fed rats. The osteoclast number in vitro were increased in the D-gal, insulin, and combined groups to a similar extent. These findings suggest that aging aggravates bone dyshomeostasis in the obese condition in a time-dependent manner.
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spelling pubmed-91231712022-05-22 D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis Imerb, Napatsorn Thonusin, Chanisa Pratchayasakul, Wasana Arunsak, Busarin Nawara, Wichwara Ongnok, Benjamin Aeimlapa, Ratchaneevan Charoenphandhu, Narattaphol Chattipakorn, Nipon Chattipakorn, Siriporn C. Sci Rep Article We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or 150 mg/kg/day of D-gal for 4 or 8 weeks. Blood was collected to measure metabolic, aging, oxidative stress, and bone turnover parameters. Bone oxidative stress and inflammatory markers, as well as bone histomorphometry were also evaluated. Additionally, RAW 264.7 cells were incubated with either D-gal, insulin, or D-gal plus insulin to identify osteoclast differentiation capacity under the stimulation of receptor activator of nuclear factor κB ligand. At week 4, D-gal-induced aging significantly elevated serum malondialdehyde level and decreased trabecular thickness in ND- and HFD-fed rats, when compared to the control group. At week 8, D-gal-induced aging further elevated advanced glycation end products, increased bone inflammation and resorption, and significantly impaired bone microarchitecture in HFD-fed rats. The osteoclast number in vitro were increased in the D-gal, insulin, and combined groups to a similar extent. These findings suggest that aging aggravates bone dyshomeostasis in the obese condition in a time-dependent manner. Nature Publishing Group UK 2022-05-20 /pmc/articles/PMC9123171/ /pubmed/35595806 http://dx.doi.org/10.1038/s41598-022-12206-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Imerb, Napatsorn
Thonusin, Chanisa
Pratchayasakul, Wasana
Arunsak, Busarin
Nawara, Wichwara
Ongnok, Benjamin
Aeimlapa, Ratchaneevan
Charoenphandhu, Narattaphol
Chattipakorn, Nipon
Chattipakorn, Siriporn C.
D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
title D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
title_full D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
title_fullStr D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
title_full_unstemmed D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
title_short D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
title_sort d-galactose-induced aging aggravates obesity-induced bone dyshomeostasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123171/
https://www.ncbi.nlm.nih.gov/pubmed/35595806
http://dx.doi.org/10.1038/s41598-022-12206-4
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